| 赤霉素对根尖径向生长的调节作用研究 |
| |
| 引用本文： | 钮世辉,李伟,陈晓阳.赤霉素对根尖径向生长的调节作用研究[J].北京林业大学学报,2013,35(3):71-76. |
| |
| 作者姓名： | 钮世辉 李伟 陈晓阳 |
| |
| 作者单位： | 1 北京林业大学生物科学与技术学院,林木育种国家工程实验室摇2 华南农业大学林学院 |
| |
| 基金项目： | 中央高校基本科研业务费专项资金 |
| |
| 摘 要： | 通过转基因与RNAi 基因沉默技术,获得了由35S 组成型启动子和TobRB7 根尖特异表达型启动子分别启动 表达的PtGA20ox、PtGA2ox1 烟草转基因株系和NtGA20ox RNAi 株系,对转基因植株根尖进行大样本(n 100)显微 观察。统计结果显示:转基因植株根尖径向生长与对照相比具有极显著差异,外源施用GA3 与赤霉素合成抑制剂 PAC 可以逆转这种差异,表明赤霉素对根尖径向生长具有负调控作用;此外,利用TobRB7 启动子在根尖分生组织 特异上调和下调内源赤霉素水平可以得到与组成型调控转基因植株相似的表型,表明赤霉素对根尖径向生长的负 调控作用主要是通过对根尖分生组织生长发育的调控实现的。
|
| 关 键 词： | 赤霉素 根径 径向生长 根尖分生组织 |
| 收稿时间： | 1900-01-01 |
Negative regulation of gibberellin on root tip diameter |
| |
| NIU Shi-hui,; LI Wei,; CHEN Xiao-yang.Negative regulation of gibberellin on root tip diameter[J].Journal of Beijing Forestry University,2013,35(3):71-76. |
| |
| Authors: | NIU Shi-hui ; LI Wei ; CHEN Xiao-yang |
| |
| Institution: | 1 National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, 100083, P. R. China; 2 College of Forestry, South China Agricultural University, Guangzhou, 510642, P. R. China. |
| |
| Abstract: | We conducted our study on tobacco plants for altered rates of biosynthesis and catabolism of GA constitutively or in specific tissues by transgenic approach and RNAi gene silencing technology. In the present study, PtGA20ox and PtGA2ox1 were over-expressed under the control of 35S promoter or root meristem-specific promoter (TobRB7) respectively and NtGA20ox was silenced. Root tips of transgenic plants and NtGA20ox RNAi lines were microscopic observation (n>100). It showed that transgenic plants compared with control had a very significant difference. This difference can be reversed by the exogenous application of GA3 and gibberellin synthesis inhibitor PAC. Moreover, similar phenotypes were obtained for root tip meristem-specific up-and down-regulation and constitutive regulation of endogenous gibberellin levels. The results provide evidence that GA negative regulates the root tip diameter through effects on the meristem. |
| |
| Keywords: | gibberellin root tip diameter radial growth root meristem |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《北京林业大学学报》浏览原始摘要信息 |
| 点击此处可从《北京林业大学学报》下载免费的PDF全文 |
|
