| 落叶松毛虫AChE 最佳反应体系建立及其 对3 种杀虫剂敏感性比较 |
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| 引用本文： | 邹传山,曹传旺,王志英.落叶松毛虫AChE 最佳反应体系建立及其 对3 种杀虫剂敏感性比较[J].北京林业大学学报,2013,35(3):102-107. |
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| 作者姓名： | 邹传山 曹传旺 王志英 |
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| 作者单位： | 东北林业大学林学院 |
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| 基金项目： | 林业公益性行业科研专项，黑龙江省科技计划项目，中央高校基本科研业务费专项资金项目 |
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| 摘 要： | 通过正交试验和酶活性测定法研究了落叶松毛虫6 龄幼虫乙酰胆碱酯酶(AChE)最佳反应体系及对3 种杀 虫剂的敏感性。结果表明:落叶松毛虫6 龄幼虫AChE 最佳反应条件为酶液质量浓度0郾2 g/ mL、底物浓度0郾8 mmol/ L、反应体系pH 7郾5、水浴温度35 益和反应时间5 min。敌敌畏、高效氯氟氰菊酯和阿维菌素对落叶松毛虫6 龄幼虫体外AChE 活性产生不同的抑制作用,且抑制效应与杀虫剂呈现明显的剂量鄄效应关系;其中敌敌畏抑制效 果最显著(IC50 =3.018 ·10 -4 mg/ mL),其次是高效氯氟氰菊酯(IC50 = 1.436 ·10 -2 mg/ mL),而阿维菌素对AChE 活性抑制效果最弱。
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| 关 键 词： | 落叶松毛虫 乙酰胆碱酯酶活性 正交试验 杀虫剂 |
| 收稿时间： | 1900-01-01 |
Determination of optimized reaction system for AChE in Dendrolimus superans and comparison of its sensitivity to three pesticides |
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| ZOU Chuan-shan; CAO Chuan-wang; WANG Zhi-ying.Determination of optimized reaction system for AChE in Dendrolimus superans and comparison of its sensitivity to three pesticides[J].Journal of Beijing Forestry University,2013,35(3):102-107. |
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| Authors: | ZOU Chuan-shan; CAO Chuan-wang; WANG Zhi-ying |
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| Institution: | School of Forestry,Northeast Forestry University,Harbin,150040,P. R. China. |
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| Abstract: | In this study, the optimal reaction system of acetylcholinesterase (AChE) in 6th instar larval Dendrolimus superans was determined by orthogonal matrix method, then sensitivities of AChE to DDVP, lambda-cyhalothrin and avermectins were compared through enzyme assay. The results showed that the optimal conditions of AChE activity assay in 6th instar larval D. superans were 0.2 g/mL of AChE concentration, 0.8 mmol/L of substrate concentration, pH 7.5, 35 ℃ of temperature and 5 minutes of reaction time. Moreover, the three pesticides, DDVP, lambda-cyhalothrin and avermectins, showed different inhibition to in vitro AChE activity in 6th instar larval D. superans. Relationship of inhibitory effect and pesticides was in a concentration way. Among three pesticides, DDVP showed the most significant inhibition to AChE activity in 6th instar larval D. superans (IC50=3.018×10-4 mg/mL), following lambda-cyhalothrin(IC50=1.436×10-2 mg/mL), whereas avermectin was the weakest. These results will provide basal information for further studying the biochemical characteristics of AChE and toxicity mechanisms of pesticides. |
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| Keywords: | Dendrolimus superans acetylcholinesterase(AChE)activity orthogonal matrix pesticide |
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