| 苹果片在干制条件下的蛋白质组学分析 |
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| 引用本文： | 李新明,李群,韩彦龙,高忠东,王峰,田志芳,徐琳,王贤萍,刘森.苹果片在干制条件下的蛋白质组学分析[J].安徽农业科学,2018,46(2):70-74,89. |
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| 作者姓名： | 李新明 李群 韩彦龙 高忠东 王峰 田志芳 徐琳 王贤萍 刘森 |
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| 作者单位： | 山西省农业科学院农产品加工研究所,山西太原,030031;山西省农业科学院果树研究所,山西太原,030800 |
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| 基金项目： | 山西省自然科学基金项目 |
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| 摘 要： | 目的]研究苹果片在干制条件下及复合护色剂处理后的蛋白质水平变化。方法]采用Label free技术分析了苹果片在干制条件下及复合护色剂处理后果肉中蛋白质组学的变化。结果]一共检测到319个差异蛋白上调,590个下调,分别参与了多个不同的代谢途径。其中,催化活性catalytic activity(48个差异表达蛋白)、氧化还原过程oxidation-reduction process(18个差异表达蛋白)、应激反应response to stress(17个差异表达蛋白)等多个途径的蛋白差异表达明显。KEGG富集分析结果显示,共有2个基因通路显著富集,在氧化磷酸化Oxidative phosphorylation中有5个蛋白参与显著富集;在代谢通路Metabolic pathways中有11个蛋白参与显著富集。这2个途径与抗氧化、褐变酶合成途径有关。结论]该研究首次在苹果片干制条件下及复合护色剂处理后采用蛋白质组学的方法分析差异蛋白的表达变化,为复合护色剂在实践中果蔬护色提供科学依据。
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| 关 键 词： | 复合护色剂 苹果片 干制 蛋白质组学 表达分析 GO富集分析 |
Proteomic Studies in Apple Slices during Drying |
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| Abstract: | Objective] To investigate proteins expression in complex colour-protective reagents treated apple slices during drying.Method] We analyzed proteomic change in apple slices treated with complex colour-protective reagents during drying using Label free technology.Result]319 differential proteins were upregulated,590 differential proteins were downregulated.They involved into different metabolism pathways,respectively.Among them,differential proteins expression in catalytic activity (48 proteins),oxidation-reduction process (18 proteins),response to stress (17 proteins) pathways became very obvious.KEGG enrichment analysis showed that two gene pathways were obviously enriched.5 proteins involved into Oxidative phosphorylation pathway enrichment.11 proteins involved into metabolic pathways enrichment.The two pathways were related to antioxidation and enzymes browning procedures.Conclusion] Proteomic change in complex colourprotective reagents treated apple slices during drying was first reported.This study provided a science support to application of complex colourprotective reagents in fruits and vegetables browning inhibition. |
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