| 2016年江苏省部分猪场猪伪狂犬病原学监测与变异性分析 |
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| 引用本文： | 袁朗,胡金成,张敬峰,张晓曦,孙华伟.2016年江苏省部分猪场猪伪狂犬病原学监测与变异性分析[J].安徽农业科学,2018,46(1):91-93. |
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| 作者姓名： | 袁朗 胡金成 张敬峰 张晓曦 孙华伟 |
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| 作者单位： | 江苏省农业科学院兽医研究所,江苏南京,210014;南京农业大学动物医学院,江苏南京,210095 |
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| 基金项目： | 江苏省科技成果转化与推广资金项目 |
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| 摘 要： | 目的]研究猪伪狂犬病毒在江苏省的遗传变异情况.方法]对2016年1-12月江苏不同地区42家规模猪场采集或者送检的共2365份病料组织进行PCR检测,分析不同季节、猪场规模以及地区的检测结果,并把检测出的部分基因序列进行同源性分析.结果]猪伪狂犬阳性样品364份,阳性率为15.4％;夏、秋两季的病原阳性率均低与春、冬两季;苏北以及苏中地区的病原阳性率高于苏南地区;不同规模的猪场病原阳性率随着猪场规模的扩大逐渐下降且趋势明显.11株分离株中PRV-6分离株与Barthak61疫苗株属于同一个分支.结论]该研究可为江苏省乃至全国猪伪狂犬病的防控提供参考.
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| 关 键 词： | 猪伪狂犬病毒 病原学 监测 变异性 |
Pseudorabies Pathogen Monitoring and Variability Analysis of Part of Pig Farm in Jiangsu in 2016 |
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| Abstract: | Objective]The aim is to study genetic variation of rabies virus in Jiangsu Province.Method] PVR detection of 2365 histopathology sampling and submittal for inspection from 42 large-scale pig farms was carried out in Jiangsu area during Jan.-Dec.2016.Test result was analyzed in different seasons,large-scale pig farms and areas.Homology of determined part of gene sequence was analyzed.Result] Male samples of Porcine pseudorabies were 364.Positive rate was 15.4％.Positive rate of pathogen in summer and autumn was higher than that of winter and spring.Positive rate of Northern Jiangsu and Central Jiangsu Region was higher than that of Southern Jiangsu.Positive rate of pathogen decreased with expanding large-scale pig farms.PRV-6 isolates of 11 isolates and Barthak61 Vaccine strain belong to the same branch.Conclusion] The research can provide reference for preventing and controlling Porcine pseudorabies of Jiangsu and the whole country. |
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