| 猕猴桃胚乳培养研究 |
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| 引用本文： | 穆瑢雪,赵丹丹,刘永立.猕猴桃胚乳培养研究[J].安徽农业科学,2018,46(1):59-60. |
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| 作者姓名： | 穆瑢雪 赵丹丹 刘永立 |
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| 作者单位： | 浙江大学农业与生物技术学院,浙江杭州,310058;浙江大学农业与生物技术学院,浙江杭州,310058;浙江大学农业与生物技术学院,浙江杭州,310058 |
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| 基金项目： | 浙江省科技厅科技攻关重大农业项目“猕猴桃分子育种研究” |
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| 摘 要： | 目的]建立猕猴桃胚乳培养体系。方法]将猕猴桃种子消毒后取胚乳作为外植体,接种于含有NAA和6-BA不同组合的1/2MS培养基上进行培养,调查不同NAA和6-BA组合对猕猴桃不同器官形成的影响。结果]6-BA对不定芽的形成有促进作用,其中植物生长调节剂组合NAA 0.1μmol/L+6-BA 5μmol/L对不定芽的形成最有效,而植物生长调节剂组合NAA 0.5μmol/L+6-BA1μmol/L对不定根的形成最有效。结论]该研究为猕猴桃的胚乳培养提供了技术支持。
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| 关 键 词： | 猕猴桃 胚乳培养 植物生长调节剂 不定芽 不定根 |
Study on in vitro Culture of Kiwifruit Endosperm |
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| MU Rong-xue,ZHAO Dan-dan,LIU Yong-li.Study on in vitro Culture of Kiwifruit Endosperm[J].Journal of Anhui Agricultural Sciences,2018,46(1):59-60. |
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| Authors: | MU Rong-xue ZHAO Dan-dan LIU Yong-li |
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| Abstract: | Objective] To establish in vitro culture system of kiwifruit endosperm.Method] After disinfection of the seeds of kiwifruit,the endosperm was used as explant to inoculate on 1/2MS medium containing different combinations of NAA and 6-BA.We investigated the effects of different 6-BA and NAA combinations on the organogenesis of kiwifruit endosperm callus tissue.Result]6-BA preferably promote organogenesis of endosperm callus.The maximum induction frequency of the adventitious buds was observed in the combination of 0.1 μmol/L NAA +5 μmol/L 6-BA,while the maximum induction frequency of the adventitious roots was observed in the combination of 0.5 μmol/L NAA + 1 μmol/L 6-BA.Conclusion] This study provided technical support for kiwifruit endosperm culture. |
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