| 细粒棘球绦虫原头节及囊壁的蛋白质,糖,脂及酯酶同工酶分析 |
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| 引用本文： | 朱兴全,叶炳辉.细粒棘球绦虫原头节及囊壁的蛋白质,糖,脂及酯酶同工酶分析[J].畜牧兽医学报,1994,25(2):150-153. |
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| 作者姓名： | 朱兴全 叶炳辉 |
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| 作者单位： | 中国农业科学院兰州兽医研究所，南京医学院 |
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| 摘 要： | 本试验应用聚丙烯酰胺凝胶等电聚焦电泳时对细粒棘球绦虫原头节及囊壁可溶性粗提物的蛋白质，糖，脂及酯酶同工酶进行了初步分析。结果表明，用考马斯亮兰Ｒ－２５０染蛋白分别显示１８及１７条带，用ＰＡＳ试剂染糖分别显示４及８条带；用Ｎｉｌｅｓ兰染脂分别显示４及带；用坚固兰染酯酶同工酶，分别显示５及１０条带。两者的蛋白组成包括酸性糖蛋白，酸性脂蛋白，酸性糖脂蛋白及单纯蛋白。多糖均为酸性。
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| 关 键 词： | 细粒棘球绦虫 原头节 囊壁 蛋白 |
ANALYSIS OF PBOTEINS,POLYSACCHABIDES, LIPIDS ANDESTEBASE ISOENZYME OF THE PROTOSCOLEXAND CAPSULE WALL |
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| Zhu Xingquan,Dou Lanqing, Shi Xiaohong,Sun Xueqin,Niu Bingheng.ANALYSIS OF PBOTEINS,POLYSACCHABIDES, LIPIDS ANDESTEBASE ISOENZYME OF THE PROTOSCOLEXAND CAPSULE WALL[J].Acta Veterinaria et Zootechnica Sinica,1994,25(2):150-153. |
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| Authors: | Zhu Xingquan Dou Lanqing Shi Xiaohong Sun Xueqin Niu Bingheng |
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| Abstract: | The proteins, polysaccharides,Iipids and esterase isoenzyme of the Protoscolex andcapsule wall of Echinococcus granulosus were analysed by isoelectric focusing (IEF) elec-trophoresis. The results indicate that the proteins, polysaccharides, lipids and esteraseisoenzymes show 18,4,4 and 5 in the Protoscolex;17,8,3 and 10 in the capsule wall.The proteins of both protoscolex and capsule wall include acid glycoproteins, acid lipid-Proteins and acid glycolipidproteins. All the Polysaccharides were acid. |
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| Keywords: | Protoscolex Capsule wall Protein Lipid Polysaccharide EsteraseIsoenzyme IEF |
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