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不同贮存条件下野坝子蜜淀粉酶活性随时间的变化规律研究
引用本文:范路军,张永云,颜平萍,张颖,贺珠婷,李卫真,和绍禹.不同贮存条件下野坝子蜜淀粉酶活性随时间的变化规律研究[J].家畜生态学报,2012,33(6):59-62.
作者姓名:范路军  张永云  颜平萍  张颖  贺珠婷  李卫真  和绍禹
作者单位:云南农业大学动物科学技术学院;云南农业大学东方蜜蜂研究所;云南农业大学农科专业基础实验教学示范中心
基金项目:现代农业产业技术体系(蜜蜂)(CARS-45-ksj14)
摘    要: 采集封盖野坝子蜜,分装后分设阳光照射、室温见光、室温避光、冷藏和冷冻等5种条件下贮存,并分别于第1、5、10、15、20、30、60和90 d ,采用分光光度法测定蜂蜜中淀粉酶活性。结果显示,新鲜的封盖野坝子蜜淀粉酶活性为(20.848±1.121) U/g,在不同的贮存条件下贮存三个月,,阳光照射组、室温见光组、室温避光组和冷藏组的淀粉酶活性显示出相似的变化规律,即先上升后下降;冷冻组淀粉酶活性下降不显著(P>0.05)。五种贮存条件对淀粉酶活性的影响由小到大依次为冷冻、冷藏、室温避光、室温见光和阳光照射,表明蜂蜜的最佳贮存条件为冷冻保存,冷藏保存应控制在3个月以内为宜.

关 键 词:野坝子蜜  淀粉酶  贮存条件  贮存时间
收稿时间:2012-06-28

Variation of Different Storage Conditions on Amylase Activity of Elsholtzia Rugulosa Honey With Time
FAN Lu-jun,ZHANG Yong-yun,YAN Ping-pin,ZHANG Ying,HE Zhu-ting,LI Wei-zhen,HE Shao-yu.Variation of Different Storage Conditions on Amylase Activity of Elsholtzia Rugulosa Honey With Time[J].Acta Ecologae Animalis Domastici,2012,33(6):59-62.
Authors:FAN Lu-jun  ZHANG Yong-yun  YAN Ping-pin  ZHANG Ying  HE Zhu-ting  LI Wei-zhen  HE Shao-yu
Institution:1.College of Animal Science and Technology,Yunnan Agriculture University,Kunming,Yunnan 650201,China; 2.Apis Cerana Research Institute,Yunnan Agriculture University,Kunming Yunnan 650201,China; 3.Demonstration Center of Basic Experiments Teaching for Agriculture Majors,Yunnan Agriculture University,Kunming,Yunnan 650201,China)
Abstract:Capped Elsholtzia rugulosa honey was collected and placed in 5 kinds of conditions, which were in sunlight, normal temperature exposing to light, normal temperature avoiding light, refrigeration at 4 ℃ and freezing at -20 ℃. And then the method of spectrophotometry was used to determine amylase activity on the 1 st, 5 th, 10 th, 15 th, 20 th, 30 th, 60 th and 90 th days. The results showed that the amylase activity of fresh capped Elsholtzia rugulosa honey was 20.848±1.121 U/g, and stored for three months in different storage conditions, in sunlight group, normal temperature exposing to light, normal temperature avoiding light, refrigeration at 4 ℃ showed a similar curve, that was increased first and then decreased; there was no significant difference between each time in freezing group (P> 0.05). Effect of five storage conditions on amylase activity from lower to higher were freezing at -20 ℃, refrigerated at 4 ℃, normal temperature avoiding light, normal temperature exposing to light and in sunlight,revealing that the best storage condition is freezing at -20 ℃,and it is suggested to refrigerate it within three months so as to maximize its properties.
Keywords:elsholtzia rugulosa honey  amylase  storage conditions  storage time
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