| 崇化大梨×新世纪梨F1代分子遗传图谱的构建及两个果实性状的QTL分析 |
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| 引用本文： | 王龙,杨健,王苏坷,李秀根.崇化大梨×新世纪梨F1代分子遗传图谱的构建及两个果实性状的QTL分析[J].分子植物育种,2011,9(4):462-467. |
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| 作者姓名： | 王龙 杨健 王苏坷 李秀根 |
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| 作者单位： | 中国农业科学院郑州果树研究所,郑州,450009 |
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| 基金项目： | 国家863计划(2006AA100108); 国家现代梨产业技术体系(nycytx-29)共同资助 |
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| 摘 要： | 利用崇化大梨×新世纪梨杂交得到的94株F1代实生苗为作图群体,应用mapmaker/exp 3.0软件构建了一张包括19个SSR标记,315个SRAP标记,合计335个标记分属于18个连锁群的遗传图谱,图谱总长度为1 300 cM,平均图距为3.9 cM。采用区间作图法检测到与果实发育期、果形指数两个果实性状相连锁的QTL位点7个,其中主效的QTL位点2个(LOD≥3.5),分别位于LG2和LG17连锁群。
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| 关 键 词： | 梨 遗传图谱 QTL分析 果实发育期 果形指数 |
Construction of a Genetic Linkage Map and QTL Analysis of Two Fruit Traits in F1 Population of 'Chonghuadali'×'Shinseiki' |
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| Wang Long,Yang Jian,Wang Suke,Li Xiugen.Construction of a Genetic Linkage Map and QTL Analysis of Two Fruit Traits in F1 Population of 'Chonghuadali'×'Shinseiki'[J].Molecular Plant Breeding,2011,9(4):462-467. |
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| Authors: | Wang Long Yang Jian Wang Suke Li Xiugen |
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| Institution: | Wang Long Yang Jian Wang Suke Li Xiugen Zhengzhou Fruit Research Institute CAAS,Zhengzhou,450009 |
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| Abstract: | A genetic linkage map was constructed from a F1 population comprising 94 seedlings derived from a cross between ’Chonghuadali’×’Shinseiki’ using mapmaker/exp 3.0.The map consisted of 19 SSR markers and 315 SRAP markers which belong to 18 linkage groups and spans 1 300 cM with an average genetic distance of 3.9 cM.The interval mapping was used to identify quantitative trait loci(QTL) associated with fruit developing period and fruit shape index in the F1 population.The results indicated 7 QTLs were linked to the two fruit traits,of which 2 QTLs were major effect genes(LOD≥3.5).The two QTL regions were on LG2 and LG17. |
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| Keywords: | Pear Linkage map QTL analysis Fruit developing period Fruit shape index |
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