| 转IPT基因油菜提高抗蚜虫能力 |
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| 引用本文： | 李海林,赵德刚.转IPT基因油菜提高抗蚜虫能力[J].分子植物育种,2011,9(3):343-349. |
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| 作者姓名： | 李海林 赵德刚 |
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| 作者单位： | 贵州大学生命科学学院;贵州大学贵州省农业生物工程重点实验室;贵州大学教育部绿色农药与农业生物工程重点实验室; |
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| 基金项目： | 科技部国际科技合作项目(2007DFA31260)、科技部国家科技支撑计划项目(2007BAD59B06)和国家转基因新品种培育重大专项子项目（2008ZX08010-003）共同资助 |
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| 摘 要： | 本研究利用农杆菌介导IPT(isopentenyl-transferases,异戊烯基转移酶)基因转化喀斯特特有甘蓝型油菜自交品种S65,在无激素的筛选培养基中获得再生植株14株,PCR检测12株外源基因整合入油菜基因组中.对其中5株转基因植株及5株野生型植株人工接种蚜虫试验,研究超量表达IPT基因油菜对蚜虫的抗性.结...
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| 关 键 词： | IPT基因 转基因油菜 蚜虫 人工接虫 |
IPT Gene Expression Increased the Aphid-resistant of Transgenic Plant in Oilseed |
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| Li Hailin,Zhao Degang.IPT Gene Expression Increased the Aphid-resistant of Transgenic Plant in Oilseed[J].Molecular Plant Breeding,2011,9(3):343-349. |
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| Authors: | Li Hailin Zhao Degang |
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| Institution: | Li Hailin 1,2 Zhao Degang 1,2,3 1 College of Life Sciences,Guizhou University,Guiyang,550025,2 Guizhou Key Laboratory of Agro-Bioengineering,3 Ministry of Education Key Laboratory of Green Pesticide and Ago-Bioengineering |
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| Abstract: | In this study, Karst unique inbred strains oilseed (Brassica napus L.) S65 was transformed by Agrobacterium-mediated method with the plasmids DNA containing IPT gene,14 regenerated plants were obtained from hormone-free selection medium, and IPT gene was integrated into the genomes of 12 plants by PCR identification. The aphids-resistant of over-expression IPT gene oilseed were studied by the artificial inoculation aphids on. The result showed that transgenic oilseed at aphid-resistant was more stronger tha... |
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| Keywords: | IPT gene Transgenic oilseed Aphid Artificial inoculation |
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