| 苹果高效遗传转化受体系统的建立与优化 |
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| 引用本文： | 雷蓉华,马锋旺,梁东.苹果高效遗传转化受体系统的建立与优化[J].西北农业学报,2007,16(6):128-132. |
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| 作者姓名： | 雷蓉华 马锋旺 梁东 |
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| 作者单位： | 西北农林科技大学园艺学院,陕西杨凌,712100 |
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| 基金项目： | 陕西省重大科技专项计划;西北农林科技大学校科研和教改项目 |
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| 摘 要： | 以嘎拉（Gala）和长富2号（Nagafu No.2）组培苗离体叶片为外植体,进行了不同生长调节剂浓度、叶片成熟度、暗培养时间、叶片极性及抗生素筛选研究。结果表明,嘎拉叶片适宜的再生培养基为MS＋TDZ0.5 mg/L＋NAA 0.4 mg/L,叶片近轴面接触培养基;长富2号叶片适宜的培养基为MS＋TDZ 4.0 mg/L＋NAA 0.6 mg/L,叶片远轴面接触培养基。经过21 d的暗培养后,嘎拉和长富2号叶片再生频率分别可达100%和60%。抗生素筛选浓度为卡那霉素5 mg/L,头孢霉素200-300 mg/L。
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| 关 键 词： | 苹果 受体系统 遗传转化 不定芽 |
| 文章编号： | 1004-1389（2007）06-0128-05 |
| 收稿时间： | 2007-04-05 |
| 修稿时间： | 2007-04-29 |
Establishment and Optimization of Acceptor System with High Frequency for Gene Transformation of Apple |
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| LEI Rong-hu,MA Feng-wang and LIANG Dong.Establishment and Optimization of Acceptor System with High Frequency for Gene Transformation of Apple[J].Acta Agriculturae Boreali-occidentalis Sinica,2007,16(6):128-132. |
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| Authors: | LEI Rong-hu MA Feng-wang and LIANG Dong |
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| Institution: | College of Horticulture, Northwest A & F University, Yangling Shaanxi 712100, China;College of Horticulture, Northwest A & F University, Yangling Shaanxi 712100, China;College of Horticulture, Northwest A & F University, Yangling Shaanxi 712100, China |
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| Abstract: | Experiments of the concentration of growth hormone, maturity, dark treatment, leaf polarity and the concentration of the antibiotics were conducted with leaves of Gala and Nagafu No. 2 as in vitro explants. The results showed the Gala leaves were able to form buds on the MS medium containing 0.5 mg/LTDZ and 0.4 mg/LNAA with abaxial surface,adaixial surface of Nagafu No. 2 leaves touching MS medium with 4.0 mg/L TDZ and 0.6 mg/L NAA. After about 21d dark incubation treatment ,the generation efficiency of Gala and NagafuNo. 2 leaves reached 100% and 60%,respecticely. The concentration of Km and Cef were 5 mg/L and 200-300 mg/L. |
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| Keywords: | Apple Acceptor system Transformatiom Adventitious shoots |
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