| 鸡肾型IBV的分离及其N基因的克隆与测序 |
| |
| 引用本文： | 张建臣.鸡肾型IBV的分离及其N基因的克隆与测序[J].水禽世界,2008(9). |
| |
| 作者姓名： | 张建臣 |
| |
| 作者单位： | 山东畜牧兽医职业学院,潍坊,261061 |
| |
| 摘 要： | 通过病毒形态学观察、血凝试验、病毒干扰实验、动物回归实验等分离鉴定了1株鸡肾型传染性支气管炎病毒.利用RT-PCR技术对分离毒株的N基因进行了扩增,经克隆、序列测定和分析,证实分离株为肾型IBV.
|
| 关 键 词： | 鸡肾型传染性支气管炎病毒 分离 N基因 序列测定 |
Isolation of Avian Infectious Bronchitis Nephritis Virus and Cloning and Sequencing of the Virus N gene |
| |
| ZHANG Jian-chen.Isolation of Avian Infectious Bronchitis Nephritis Virus and Cloning and Sequencing of the Virus N gene[J].Poultry Science,2008(9). |
| |
| Authors: | ZHANG Jian-chen |
| |
| Institution: | Shandong Vocational Animal Science and Veterinary College;Weifang 261061;China |
| |
| Abstract: | One strain of avian infectious bronchitis nephritis virus(IBV) was identified by morphological observation, inoculating virus in to chicken embryos,vira1 interference test, HA test and returning pathogen to host. The N gene of the strain was amplified by RT-PCR, and it was proved to be the N gene of infectious bronchitis virus by cloning, sequencing, and analysis .The results showed that the strain isolated from the chickens was avian infectious bronchitis nephritis virus. |
| |
| Keywords: | avian infectious bronchitis nephritis virus isolation N gene sequencing |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
