Genetic variations and interrelationships in Vanilla planifolia and few related species as expressed by RAPD polymorphism |
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| Authors: | D Minoo V N Jayakumar S S Veena J Vimala A Basha K V Saji K Nirmal Babu K V Peter |
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| Institution: | (1) Biotechnology Facility, Indian Institute of Spices Research, Calicut, 673 012, Kerala, India;(2) Kerala Agricultural University, Thrissur, 680 656, Kerala, India |
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| Abstract: | Vanilla is naturally distributed in Mexico and parts of Central America and the history of origin of cultivated vanilla suggests
that the entire stock outside Mexico may be from a single genetic source. In the present study, RAPD polymorphism was used
to estimate the level of genetic diversity and interrelationships among different collections of Vanilla planifolia Andr., and few related species, including both leafy and leafless types such as V. tahitensis J.W.Moore, V. andamanica, Rolfe, V. pilifera Holtt., and V. aphylla Blume. Studies revealed that there are very limited variation within collections of V. planifolia, indicative of its narrow genetic base, and of the related species we tested, V. tahitensis is nearest to V. planifolia. The species studied are diverse and have a similarity ranging from 1.2 to 57.3 %. Of the sampled taxa, V. andamanica is the most divergent and there is also reasonable variability within its collections, indicating the possibility of natural
seed set. A total of 82 polymorphic bands expressed in the RAPD profiles were used to generate a genetic distance matrix,
which was then used in cluster analysis. Specific groupings were revealed by the cluster analysis whereby the leafless forms
(V. aphylla, V. pilifera and the new species) and V. andamanica formed separate clusters. This is the first report of species interrelationship studies, including both cultivated and wild
vanilla species. |
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| Keywords: | RAPD Species relationships Vanilla andamanica V aphylla V pilifera V planifolia V tahitensis |
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