| 泥鳅与大鳞副泥鳅群体RAPD分析 |
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| 引用本文： | 龙良启,汤宝贵.泥鳅与大鳞副泥鳅群体RAPD分析[J].华中农业大学学报,1998,17(6):566-569. |
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| 作者姓名： | 龙良启 汤宝贵 |
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| 作者单位： | 华中农业大学水产学院 |
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| 基金项目： | 国家自然科学基金资助课题 |
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| 摘 要： | 用从１００种随机引物中筛选出的４种引物对自然群体中的泥鳅，大鳞副泥鳅进行了ＲＡＰＤ分析，结果表明：４种引物（Ｓ２９，Ｓ９６，Ｓ９７，Ｓ９９）分析得到泥鳅种教员人遗传的纯度分别为０．５４４９，０．４９４６，０５９２６，０．６４２０，大鳞副泥鳅遗传纯度分别为０．４４９，０．４３０４，０．５１５９，０．５２４；两者间遗传距离依闪为０．５３８５，０．５７１４，０．５０２４，０．５２６６；泥鳅特征带１．０ｋ
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| 关 键 词： | 泥鳅 大鳞副泥鳅 随机扩增多态 DNA RAPD |
RAPD ANALYSIS ON NATURE POPULATION OF Misgurnus anguillicandatus and Paramisgurnus dabryamus |
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| Long Liangqi\ Tang Baogui\ Zhao Zhenshan\ Jian yunhan.RAPD ANALYSIS ON NATURE POPULATION OF Misgurnus anguillicandatus and Paramisgurnus dabryamus[J].Journal of Huazhong Agricultural University,1998,17(6):566-569. |
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| Authors: | Long Liangqi\ Tang Baogui\ Zhao Zhenshan\ Jian yunhan |
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| Abstract: | RAPD PCR analysis on the nature population of M. anguillicaudutus and P. debryanus was made by four random primers selected from 100 randem primers. The results showed. That genetic purity calculated by primer S 29 ,S 96 ,S 97 ,S 99 was 0.5449, 0.4941, 0.5926 and 0.6420 in M. anguillicaudatus ; 0.4449,0.4304,0.5159, and 0.5424 in P. dabryanus respecitively. Both fishes' genetic distances were 0.5385, 0.5714, 0.5024 and 0.5266 for each primer. The specific bands were 1.0kb, 0.6kb, 0.9kb, 0.2kb in M. anguillicaudatus , 0.7kb , 0.52kb, 0.65kb, 0.5kb, in P. dabryanus . |
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| Keywords: | Misgurnus anguillicandatus Paramisgurnus dabryanus randon amplification polymorphism DNA |
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