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| 基于线粒体12SrRNA序列研究凤鲚两个野生群体的遗传多样性 | |
| 引用本文： | 程起群,马春艳,缪炯,陆星,沙珍霞.基于线粒体12SrRNA序列研究凤鲚两个野生群体的遗传多样性[J].大连水产学院学报,2007,22(5):387-391. |
| 作者姓名： | 程起群 马春艳 缪炯 陆星 沙珍霞 |
| 作者单位： | 1. 中国水产科学研究院,东海水产研究所,农业部海洋与河口渔业重点实验室,上海,200090;复旦大学,生物医学研究院,上海,200032 2. 中国水产科学研究院,东海水产研究所,农业部海洋与河口渔业重点实验室,上海,200090;华东师范大学,生命科学学院,上海,200062 3. 中国水产科学研究院,东海水产研究所,农业部海洋与河口渔业重点实验室,上海,200090 4. 中国水产科学研究院,黄海水产研究所,农业部海洋渔业资源可持续利用重点实验室,青岛,266071 |
| 基金项目： | 国家自然科学基金 , 中国博士后科学基金 , 农业部重点实验室基金 |
| 摘 要： | 对凤鲚Coilia mystus长江群体和珠江群体的线粒体DNA(m itochondrial DNA,m tDNA)的12SrRNA基因片断序列进行分析。排列比对后,获得该基因365 bp的一致序列。在所测得的42个序列中,共检测到6种单倍型,其中长江群体有2种,珠江群体有4种。长江群体和珠江群体的单倍型多样性指数分别为0.5263和0.6104,核苷酸多样性指数分别为0.144%和0.192%。凤鲚长江群体内部的遗传距离为0.3%,珠江群体内部的遗传距离为0.3%～0.5%,长江群体与珠江群体之间的遗传距离为0.8%～1.4%。邻接树显示长江凤鲚和珠江凤鲚各自形成一个单系类群。AMOVA分析显示群体间的变异占总变异的82.80%,提示两者可能有相互隔离的遗传结构。 |
| 关 键 词： | 凤鲚 12SrRNA 遗传多样性 分子标记 |
| 文章编号： | 1000-9957（2007）05-0387-05 |
| 修稿时间： | 2007-03-09 |
Genetic diversity in two wild populations of tapertail anchovy Coilia mystus via 12S ribosomal segment sequences of mitochondrial DNA |
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| CHENG Qi-qun,MA Chun-yan,MIAO Jiong,LU Xing,SHA Zhen-xia.Genetic diversity in two wild populations of tapertail anchovy Coilia mystus via 12S ribosomal segment sequences of mitochondrial DNA[J].Journal of Dalian Fisheries University,2007,22(5):387-391. | |
| Authors: | CHENG Qi-qun MA Chun-yan MIAO Jiong LU Xing SHA Zhen-xia |
| Institution: | 1. Key Laboratory of Marine and Estuarine Fisheries, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China; 2. Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China; 3. School of Life Science, East China Normal University, Shanghai 200062, China; 4. Key Laboratory of Sustainable Utilization of Marine Fishery Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China |
| Abstract: | Genetic diversities in two populations of tapertail anchovy Coilia mystus,Yangtze River population and Pearl River population,were investigated by 12S ribosomal segment sequences of mitochondrial DNA.A total of forty-two individuals including twenty in Yangtze River populations and twenty-two in Pearl River population were collected.Sequences of 12S ribosomal(12SrRNA) amplified by PCR was used and 365 base pairs concensus sequences were obtained.Six different haplotypes were detected among all the 42 sequences,two in Yangtze River population and four in Pearl River population.The haplotype diversity index of Yangtze River population and Pearl River population was 0.5263 and 0.6104,respectively.The nucleotide diversity of Yangtze River population and Pearl River population was 0.144% and 0.192%,respectively.Genetic distances were found to be 0.3% in the Yangtze River population,and between 0.3% and 0.5% in the Pearl River population.The genetic distances varied from 0.8% to 1.4% between the Yangtze River population and the Pearl River population.The Neighbour-Joining tree supported the two populations forming monophyletic group respectively.Analysis of molecular variance(AMOVA) analysis revealed that the variation between the populations accounted for 82.80% in total variation,suggesting that their genetic structure be isolated each other. |
| Keywords: | Coilia mystus 12SrRNA genetic diversity molecular marker |
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