| 2012~2013年山东省禽源大肠杆菌中质粒介导喹诺酮类药物耐药基因的检测 |
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| 引用本文： | 孔令超,李志国,张瑞华,焦玉祥,马明杰,郭翠平,朱岩丽,谢之景,姜世金.2012~2013年山东省禽源大肠杆菌中质粒介导喹诺酮类药物耐药基因的检测[J].中国动物传染病学报,2014(4):56-62. |
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| 作者姓名： | 孔令超 李志国 张瑞华 焦玉祥 马明杰 郭翠平 朱岩丽 谢之景 姜世金 |
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| 作者单位： | 山东农业大学动物医学院山东省动物生物工程与疾病防治重点实验室,泰安271018 |
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| 基金项目： | 山东省现代农业产业技术体系家禽创新团队（SDAIT-13-011-15） |
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| 摘 要： | 为研究近年来山东省禽源致病性大肠杆菌中质粒介导喹诺酮类药物耐药(plasmid-mediated quinolone resistance,PMQR)基因的基因型分布,及其对喹诺酮类抗生素的耐药性的影响,分别采用针对qnrA、qnrB、qnrC、qnrD、qnrS、oqxA、oqxB与qepA 8个耐药基因的通用引物,对93株2012~2013年分离自山东省的禽源大肠杆菌进行PCR检测,并对其进行了5种喹诺酮类药物的药敏试验。结果表明山东省禽源大肠杆菌对5种喹诺酮类抗生素均产生了较高耐药性(50.54%~86.30%);PMQR基因携带率达到60.21%(56/93),其中26.88%(25/93)的菌株携带2种PMQR基因,1.07%(1/93)的菌株携带3种PMQR基因;qnrA、qnrB、qnrC、qnrD与qepA基因未被检测到,qnrS、oqxA和oqxB基因在山东省禽源致病性大肠杆菌中分布较为广泛,其检出率依次为22.58%(21/93)、40.86%(38/93)和24.73%(23/93)。
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| 关 键 词： | 禽源致病性大肠杆菌 药敏试验 质粒介导喹诺酮类药物耐药基因 PCR |
DETECTION OF PLASMID-MEDIATED QUINOLONE RESISTANCE GENES IN AVIAN ESCHERICHIACOLI ISOLATES FROM SHANDONG PROVINCE IN 2012-2013 |
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| KONG Ling-chao,LI Zhi-Guo,ZHANG Rui-hua,JIAO Yu-xiang,MA Ming-jie,GUO Cui-ping,ZHU Yan-li,XIE Zhi-jing,JIANG Shi-jin.DETECTION OF PLASMID-MEDIATED QUINOLONE RESISTANCE GENES IN AVIAN ESCHERICHIACOLI ISOLATES FROM SHANDONG PROVINCE IN 2012-2013[J].Chinese Journal of Animal Infectious Diseases,2014(4):56-62. |
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| Authors: | KONG Ling-chao LI Zhi-Guo ZHANG Rui-hua JIAO Yu-xiang MA Ming-jie GUO Cui-ping ZHU Yan-li XIE Zhi-jing JIANG Shi-jin |
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| Institution: | (Shandong Province Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Veterinary Medicine, Shandong Agriculture University, Taian 271018, China ) |
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| Abstract: | To investigate the distribution of plasmid-mediated quinolone resistance （PMQR） genes and their effect on the bacterial resistance to the quinolone antibiotics in recent years, qnrA, qnrB, qnrC, qnrD, qnrS, oqxA, oqxB and qepA genes of 93 avian E. coli strains isolated from Shandong Province were detected individually in PCR, and the resistance to Quinolones （Ciprofloxacin, Lomefloxacin, Ofloxacin, Norfloxacin, Pefloxacin） was detected in antimicrobial susceptibility test. Among 93 E.coli isolates tested, 47 （50.54%） to 80 （86.30%） strains were resistant to five Quinolones respectively, and 56 （60.21%） strains were positive to PMQR genes. In addition, 25 isolates （26.88%） harbored two kinds of PMQR genes and 1 isolates （1.07%） harbored three kinds of PMQR genes. The positive rates of qnrS, oqxA and oqxB gene were 22.58%（21/93）, 40.86%（38/93） and 24.73%（23/93） , respectively. None of qnrA, qnrB, qnrC, qnrD and qepAgenes was detected. |
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| Keywords: | Avian pathogenic Escherichia coil drug susceptibility test PMQR genes PCR |
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