| 哲罗鲑微卫星标记与重要生长性状的相关性分析 |
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| 引用本文： | 杨贵强,袁丁,马国庆,王占全,贺杰,周云.哲罗鲑微卫星标记与重要生长性状的相关性分析[J].江西农业大学学报,2016(5):968-974. |
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| 作者姓名： | 杨贵强 袁丁 马国庆 王占全 贺杰 周云 |
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| 作者单位： | 北京市水产科学研究所/国家淡水渔业工程技术研究中心,北京,100068 |
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| 基金项目： | 国家“十二五”科技支撑计划项目(2012BAD25B03)，北京市农科院青年基金项目(QNJJ201433)，北京市农科院农业科技示范推广项目(201533) Project supported by “Twelfth Five-Year Plan”of Science and Technology Support Program(2015BAD25B01)，Beijing Academy of Agriculture and Forestry Youth Fundprojects(QNJJ201433)，Promotion Program of Bei-jing Academy of Agriculture and Forestry(201533) |
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| 摘 要： | 采用10对微卫星荧光标记分析哲罗鲑Hucho taimen同池养殖群体的遗传多样性,通过SPSS13.0的GLM模型筛选出与生长性状(体质量、体长、体高和体宽)相关的微卫星标记。结果显示,10个微卫星位点的平均有效等位基因个数为2.301 4;多态信息含量0.366 6~0.557 6;观测杂合度范围0.652 2~0.989 1,均大于期望杂合度;Fis值为负数,存在杂合子过剩现象。基因座BJT4、BJT6、BJT8、BJT12、BJT18、BJT19、BJTH4和BJTH19均与体质量和体长性状极显著相关(P0.01),BJT6、BJTH6和BJTH17均与体高和体宽性状极显著相关(P0.01),发现存在"一因多效"和"多因一效"现象。不同基因型的多重比较表明,BJT12、BJT18和BJTH4基因座的AA基因型、BJT4基因座的BB基因型、BJT6基因座的AB基因型与体质量、体长呈正相关,BJTH6和BJTH17基因座的AB基因型与体高、体宽呈正相关。研究结果为哲罗鲑的分子标记辅助育种提供依据,同时也为下一步构建哲罗鲑的遗传连锁图谱提供基础数据。
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| 关 键 词： | 哲罗鲑 微卫星标记 生长性状 相关性分析 |
Analysis on the Correlation Between Microsatellite Markers and Important Growth Traits of Taimen |
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| Abstract: | The molecular markers related with growth traits ( body weight,body length and body width) of Hucho taimen were screened by the GLM model of SPSS13.0.The results show that 1)the average effective number of alleles was 2.301 4,the polymorphic information content was 0.366 6~0.557 6,the observation pop?ulation heterzygosity was from 0.3666 to 0.5576 and greater than the expected heterozygosity,the Fis value was negative,which suggested heterozygote excess.2) Loci BJT4,BJT6,BJT8,BJT12,BJT18,BJT19,BJTH4 and BJTH19 were significantly correlated with body weight and body length ( P<0.01) ,BJT6,BJTH6 and BJTH17 were significantly correlated with body height and body width ( P<0.01) ,and there was a phenomenon of“one gene can affect more than one trait” and “any trait can be affected by more than one gene”. The results of multi?comparison of different genotypes indicated that genotype AA of BJT 12,BJT 18 and BJTH4 were posi?tively correlated with body weight,body length,and genotype AB of BJTH6 and BJTH17 was positively correla?ted with body height,body width.The results of this study are valuable for marker?assisted breeding of taimen, and provide a basic data for building a genetic linkage map of taimen. |
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| Keywords: | taimen microsatellite markers growth traits correlation analysis genetic diversity |
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