| 不同冻存法对肌肉冰冻切片后HE染色和肌球蛋白ATP酶染色效果的影响 |
| |
| 引用本文： | 何茂章,张震,伍仲平,李完波,麻骏武,段艳宇.不同冻存法对肌肉冰冻切片后HE染色和肌球蛋白ATP酶染色效果的影响[J].江西农业大学学报,2016(3):519-523. |
| |
| 作者姓名： | 何茂章 张震 伍仲平 李完波 麻骏武 段艳宇 |
| |
| 作者单位： | 江西农业大学 猪遗传改良与养殖技术国家重点实验室,江西 南昌,330045 |
| |
| 基金项目： | 国家自然科学基金项目(31301950)，江西省研究生创新专项资金项目( YC2014-S191) Project supported by the National Natural Science Foundation of China(31301950)，the Special Funds for Graduate Students Innovation of Jiangxi Province(YC2014-S191) |
| |
| 摘 要： | 使用分步法和一步法冻存巴马香猪背最长肌,冰冻切片后比较苏木精-伊红染色法(HE染色)和肌球蛋白三磷酸腺苷酶染色法(ATP酶染色)效果。结果表明:分步法冻存的肌肉样品冰晶少,细胞形态完整,HE染色效果好,核质明显;ATP酶染色效果好,可较好地区分Ⅰ型Ⅱ型肌纤维。一步法冻存的肌肉样品冰晶多,细胞形态不完整,HE染色效果差,核质模糊;ATP酶染色效果差,基本染不上色,无法对肌纤维类型进行区分。分步冻存法的建立为通量式制备猪肉的恒冻切片奠定了基础,也为进一步开展相关性状的生理学、遗传学及种猪选育提供了很好的表型测定方法。
|
| 关 键 词： | 冻存方法 背最长肌 HE染色 ATP酶染色 |
Effects of Different Cryopreserving Methods on the Results of HE Staining and Myosin ATP Staning of Frozen Sections of Longissimus Dorsi |
| |
| Abstract: | In this study,the results of hematoxylin eosin staining ( HE staining) and myosin ATPase stai-ning were compared by step-by-step method and one-step method cryopreserving longissimus dorsi muscle of Bama Xiang pig.The results showed that the samples cryopreserved by step-by-step method contained less ice crystals,the cells were complete,the effect of HE staining was very good in which nucleus and cytoplasm could be observed clearly and the effect of ATP enzyme staining was good enough in which type Ⅰ and type Ⅱ muscle fibers could be distinguished easily;the samples cryopreserved by one-step method contained a large amount of ice crystals cells were broken,the effect of HE staining was very poor in which nucleus and cytoplasm could not be observed,and type Ⅰ and type Ⅱ muscle fibers could not be distinguished since myosin ATPase could hardly be stained on the frozen sections.The establishment of step-by-step cryopreserving method laid the foun-dation for preparing large number of frozen sections of pork and provided a good way for penotype indentifica-tion for further analysis of physiology,genetics and breeding. |
| |
| Keywords: | cryopreservation method longissimus dorsi HE staining ATP staining |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
