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高效松材线虫病预防注射药剂配制与林间药效研究
引用本文:来燕学,何月秋,池树友,王亚红,张毅丰,柳建定,沈幼莲,李国平,林茂松.高效松材线虫病预防注射药剂配制与林间药效研究[J].江西农业大学学报,2016(5):871-878.
作者姓名:来燕学  何月秋  池树友  王亚红  张毅丰  柳建定  沈幼莲  李国平  林茂松
作者单位:1. 浙江省宁波市森林病虫防治检疫站,浙江 宁波,315010;2. 宁波城市职业技术学院 资源环境系,浙江 宁波,315211;3. 浙江省余姚市森林病虫防治检疫站,浙江 余姚,315402;4. 浙江省慈溪市森林病虫防治检疫站,浙江 慈溪,315300;5. 浙江省奉化市森林病虫防治检疫站,浙江 奉化,315500;6. 南京农业大学 植保学院,江苏 南京,210037
基金项目:国家高技术研究发展计划(863计划)项目(2012AA101503),宁波市科技局计划项目(2003C10067) Project supported by the National High Technology Research and Development Program of China 2012AA101503),Ningbo Government Foundation for Science and Technology(2003C10067)
摘    要:为有效防治松材线虫病,采用阿维菌素和甲维盐为主药,吐温等为表面活性剂,复配混合药剂;同时,模制长喙50 m L容积药瓶,组合成适合向松树注输药物的专用注射针剂,在松材线虫病发生林地开展树干注入药剂抗松材线虫病试验。林间抗松材线虫病试验,采用2种方法进行,即树干注入药剂后作人工接种病原松材线虫和自然感染病原松材线虫;同时,用日本专用松材线虫病预防注射剂作对比试验。结果表明,日本药物(A类药)对人工接种病原松材线虫具有75%的预防效果;自配的阿维菌素(C21类药)和甲维盐(C22类药)对人工接种获得50%和100%的预防效果,使自然感染松材线虫病的松树死亡率平降至2.70%;而对照试验的人工接种病线虫的松树死亡率为100%,自然感染松树死亡率为22.36%。结果表明:试验开发的松树注射剂阿维菌素C21和甲维盐C22,能有效预防松树个体的松材线虫病发生与危害。

关 键 词:松材线虫  甲维盐  阿维菌素  树干注射  预防  接种  感染

Concoction of Two High Effect Anti-nematode Drugs Injected into Pine Tree for Prevention of Pine Wilt Disease and Their Effect in Forest
Abstract:In order to improve the control effect of pine wilt disease caused by Bursaphelenchus xylophilus, three types of injections of anti?nematode drugs contained in bottle injector were developed and a field study was conducted using these novel anti?nematode injections into pine trees for prevention of pine wilt disease caused by pathogen B. xylophilus infected naturally and inoculated artificially.The results showed that 75% of pine trees trunk?injected with the Japan?specific injection drugs ( avermectin,A type) survived from inocula?tion of B. xylophilus.The prevention effect of C21 drugs ( Abamectin+Tweens) reached 50% in the test pines inoculated with pathogen B. xylophilus,and 100% in the test pines was infected with pathogen;and that of C22 drugs( Emectin+Tweens) reached 100% in the test pines inoculated with pathogen B. xylophilus,and 96.36%in the test pines infected with pathogen.The conclusion is that the developed injection drugs (C2) can prevent PWD effectively.The preventive effect of C22 drug on PWD is better than that of Japan?specific injection drugs.
Keywords:Bursaphelenchus xylophilus  abamectin  emectin  trunk-injection  prevention  inoculation  infection
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