| 玉龙雪山不同海拔急尖长苞冷杉遗传多样性分析 |
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| 引用本文： | 程小毛,林开文,姜永雷,杨自云,黄晓霞.玉龙雪山不同海拔急尖长苞冷杉遗传多样性分析[J].江西农业大学学报,2016(4):654-659. |
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| 作者姓名： | 程小毛 林开文 姜永雷 杨自云 黄晓霞 |
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| 作者单位： | 1. 西南林业大学 园林学院,云南 昆明,650224;2. 中国科学院 成都山地灾害与环境研究所/ 山地表生过程与生态调控重点实验室,四川 成都,610041 |
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| 基金项目： | 国家自然科学青年基金项目(31100292)，云南省自然科学基金(2010ZC267)，云南省省级重点学科园林植物与观赏园艺建设经费项目(50097401) Project supported by the National Natural Science Foundation of China(31100292)，the Natural Science Founda-tion of Yunnan Province(2010ZC267)，the Construction Foundation of Provincial Key Discipline “Garden Plant and Ornamental Horticulture” of Yunnan Province(50097401) |
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| 摘 要： | 利用4对SSR引物对玉龙雪山内5个海拔梯度的急尖长苞冷杉居群进行遗传多样性和遗传结构的研究。在物种水平上,Shannon信息指数(I)和Nei基因多样性(He)分别为0.971 9和0.556 7,表现出较高的遗传多样性;玉龙雪山不同海拔梯度上急尖长苞冷杉居群的遗传多样性有差异,且随着海拔梯度的递增,居群的遗传多样性除海拔3 350 m外,其他居群的遗传多样性随海拔的增加而增加,并在海拔3 700 m处达到最大值;急尖长苞冷杉居群间的基因流(N_m)为4.778 0,群体分化系数(Fst)为0.049 7,且基于AMOVA软件分析结果显示有3.55%的变异发生在居群间,表明急尖长苞冷杉群体间分化低,且大部分变异存在于居群内。研究结果表明海拔梯度对急尖长苞冷杉遗传多样性影响甚微。
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| 关 键 词： | 急尖长苞冷杉 海拔梯度 遗传多样性 遗传分化 SSR |
Genetic Diversity of Abies georgei var. smithii at Different Altitudes in Jade Dragon Snow Mountain |
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| Abstract: | 4 pairs of SSR markers were used to study the genetic diversity and genetic structure of 5 Abies georgei var.smithii populations at different altitudes in Jade Dragon Snow Mountain in the northwest of Yunnan Province.At the species level,Shannon information index (I) and Nei’s gene diversity (He) were 0.971 9 and 0.556 7,respectively,indicating that this species has high genetic diversity at the species level.Along the altitu?dinal gradients,the genetic diversity increased with the increase of elevation gradient, except for the altitude 3 550 m. The genetic diversity of the population at altitude 3 700 m was much higher than that of other popula?tions.And the gene flow Nm was 4.778 0.AMOVA analysis showed that only 3.55% of the total genetic variation occurred among populations,whereas 96.45% of the variance was within populations,which was in line with the coefficient of genetic differentiation (Fst = 0.049 7).The results indicated that the altitude gradient had lit?tle effect on the genetic diversity of Abies georgei var. smithii. |
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| Keywords: | Abies georgei var smithii altitude genetic diversity genetic differentiation SSR |
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