| 劣味乳菇液体发酵工艺优化及其防治杨树溃疡病研究 |
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| 引用本文： | 张扬,叶建仁,吴小芹.劣味乳菇液体发酵工艺优化及其防治杨树溃疡病研究[J].江西农业大学学报,2016(6):1161-1166. |
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| 作者姓名： | 张扬 叶建仁 吴小芹 |
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| 作者单位： | 1. 南京林业大学 林学院/江苏省有害生物入侵预防与控制重点实验室，江苏 南京 210037; 江西农业大学 林学院，江西 南昌 330045;2. 南京林业大学 林学院/江苏省有害生物入侵预防与控制重点实验室,江苏 南京,210037 |
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| 基金项目： | 国家林业公益性行业科研专项(201004061) Project supported by State Forestry Industry of Public Projects(201004061) |
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| 摘 要： | 为提高菌根真菌液体发酵菌丝体生物量,以外生菌根真菌劣味乳菇(Lactarius insulsus)为试验材料,采用液体发酵法,分析接种量、溶解氧及p H值等因子对其发酵培养的影响,并将发酵产物制成液体菌剂,采用灌根法对杨树溃疡病进行田间防治试验。结果表明,接种量5%,发酵过程中p H保持5左右,发酵中后期保持溶氧浓度在28%~35%可有效提高劣味乳菇菌丝体生物量,缩短了发酵周期;田间防治试验表明,劣味乳菇对溃疡病有较好防治效果,防效可达41.1%。该研究可为该菌株作为生防菌株推广应用提供参考依据和理论基础。
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| 关 键 词： | 劣味乳菇 发酵工艺 溃疡病 防效 |
The Liquid Fermentation Technology for Lactarius insulsus and Its Control Effect on Poplar Canker Disease |
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| Abstract: | To improve the mycelia biomass of mycorrhizal fungi by liquid fermentation,with ECM Lactarius insulsus as experimental material,through the liquid fermentation,the impacts of different concentrations of dis-solved oxygen,pH and the inoculation amount on mycelial biomass were analysed, and liquid inoculation a-gainst poplar canker was conducted by using the root irrigation method.The results showed that 5% of the inoc-ulum amount,fermentation in the late dissolved oxygen concentration about 28%-35%, medium maintaining the pH about 5 can effectively improve the mycelial biomass of Li, and shorten the fermentation period. The control effect of Xerocomus chrysenteron against poplar canker reached 41.1% in fields.This study provides the reference and theoretical basis for the industrial liquid fermentation of this strain and its application as a bio-control strain. |
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| Keywords: | Lactarius insulsus fermentation technology canker control effect |
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