| 精子载体转基因的研究进展 |
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| 引用本文： | 李海华,陈英,林树茂.精子载体转基因的研究进展[J].中国畜牧兽医,2002,29(5):30-31. |
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| 作者姓名： | 李海华 陈英 林树茂 |
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| 作者单位： | 1. 江西农业大学动科院,南昌,330045
2. 中国农科院畜牧所,北京,100094 |
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| 基金项目： | 江西省自然科学基金资助项目，编号 :0 13 0 0 3 9 |
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| 摘 要： | 精子载体法培养转基因动物是目前操作最简便，成本低廉且危险隐患相对较低的一种转基因方法。近10年来的研究结果表明，精子载体法可得到转基因阳性动物，并且引起了更多科研人员的关注。
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| 关 键 词： | 精子载体 转基因 动物生产 |
| 文章编号： | 1671-7236(2002)05-0030-02 |
The Review of Sperm-Mediated Gene Transfer Research |
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| LI Hai,hua et al.The Review of Sperm-Mediated Gene Transfer Research[J].China Animal Husbandry & Veterinary Medicine,2002,29(5):30-31. |
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| Authors: | LI Hai hua |
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| Abstract: | During near decades along with the development of biotechnology, many people focus on the technology of culturing transgenic animals. The classic methods of foreign gene transfer are microinjection, retroviral vectors, chimera and so on. But high cost, complicated operation and hidden danger from these methods frustrate many researchers. Sperm mediated,gene transformation is the most convenient way and also has little concealed damage. Through the sperm mediation, some researchers transfer the DNA directly,others use liposome to introduces the foreign gene, and the rest utilize the electroporation to help the gene to transfer. All these methods are reported that they can obtain the positive animals. Nowadays more and more scientists have paid much attention to the sperm mediated gene transfer.This paper introduces the process of the research. |
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| Keywords: | Sperm mediation Gene transfer |
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