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大豆Kunitz型胰蛋白酶抑制剂(SKTI)研究进展
引用本文:赵洪锟,李启云,王玉民,庄炳昌.大豆Kunitz型胰蛋白酶抑制剂(SKTI)研究进展[J].大豆科学,2002,21(3):218-222.
作者姓名:赵洪锟  李启云  王玉民  庄炳昌
作者单位:吉林省农业科学院吉林省生物技术重点开放实验室,公主岭,136100
摘    要:大豆Kunitz型胰蛋白酶抑制剂(SKTI)是一种典型的丝氨酸蛋白酶抑制剂,主要集中在大豆的子叶中,近60年来,对其生化特性及结构已有较多研究,通常认为这种蛋白酶抑制剂具有贮藏,调节内源蛋白酶活性及植物防御等作用,研究表明,这种蛋白酶抑制剂抗虫谱广,能显著抑制幼虫的生长发育不易产生抗性,对人畜无害,因此对其进行研究及开发利用具有重要的经济和社会效益。迄今为止,至少已有4种基因被克隆,通过转基因技术在烟草,水稻,小麦等作物获得了抗性植株,本文从SKTI的类型,基因克隆及生理功能等方面作一简单综述。

关 键 词:类型  基因克隆  生理功能  分布频率  大豆  Kunitz型胰蛋白酶抑制剂  SKTI
文章编号:1000-9841(2002)03-0218-05

THE PRONGRESS OF THE STUDIES ON SOYBEAN KUNITZ TRYPSIN INHIBITOR
Zhao Hongkun,Wang Yumin,Li Qiyun,Zhuang Bingchang.THE PRONGRESS OF THE STUDIES ON SOYBEAN KUNITZ TRYPSIN INHIBITOR[J].Soybean Science,2002,21(3):218-222.
Authors:Zhao Hongkun  Wang Yumin  Li Qiyun  Zhuang Bingchang
Abstract:Kunitz trypsin inhibitor, an abundant soybean seed protein, is specific for serine proteases. It has been proposed that trypsin inhibitors are storage proteins, regulators of endogenous proteinases during seed maturation and germination, or protectors of plants against insects and microorganism by biochemistry and structure studied in the past sixty years. And it has extensive resistance to inhibit the larva development with little accumulating tolerance, so there is a significant economic and social benefit to study and apply it in production. There are at least 4 genes which have been isolated and introduced into tobacco, rice and wheat etc, now transgenic plants were obtained with resistance. In this paper, the variants, gene cloning and physiological functions were reviewed.
Keywords:Soybean Kunitz trypsin inhibitor  Variants  Gene cloning  Physiological functions
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