| 燕麦愈伤组织诱导和分化再生影响因素的研究 |
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| 引用本文： | 贾利敏,傅晓峰,孙国琴,孙瑞芬,斯钦巴特尔,刘力平,刘俊清.燕麦愈伤组织诱导和分化再生影响因素的研究[J].华北农学报,2006,21(4):31-34. |
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| 作者姓名： | 贾利敏 傅晓峰 孙国琴 孙瑞芬 斯钦巴特尔 刘力平 刘俊清 |
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| 作者单位： | 1. 内蒙古农牧业科学院生物中心,内蒙古,呼和浩特,010031
2. 内蒙古农牧业科学院甜菜研究所,内蒙古,呼和浩特,010031 |
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| 基金项目： | 内蒙古科技厅科技攻关项目 |
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| 摘 要： | 以燕麦3个品种(系)幼胚、幼穗为材料,通过组织培养方法,研究了基因型、培养基、外植体对愈伤组织诱导、继代和分化再生的影响。结果表明,对愈伤组织的诱导,基因型和培养基起重要作用,对幼胚作用极显著,对幼穗作用显著;外植体不同也影响愈伤组织形成,随培养基成分改变而变化,且幼穗较幼胚更易培养;2,4-D浓度影响愈伤组织生长和胚性愈伤组织形成,3 mg/L 2,4-D有利于愈伤生长,促进胚性愈伤形成;草莜一号幼穗愈伤组织有很强的继代能力,继代培养330 d仍具有46.58%的分化率,该材料在组织培养和基因工程研究中具有很大潜力。
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| 关 键 词： | 燕麦 幼胚 幼穗 组织培养 |
| 文章编号： | 1000-7091（2006）04-0031-04 |
| 收稿时间： | 2006-01-05 |
| 修稿时间： | 2006-01-05 |
The Studies on the Factors Affecting the Callus Induction and Plant Regeneration of Oat (Avena nuda L) |
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| JIA Li-min,FU Xiao-feng,SUN Guo-qin,SUN Rui-fen,SI Qin Bateer,LIU Li-ping,LIU Jun-qing.The Studies on the Factors Affecting the Callus Induction and Plant Regeneration of Oat (Avena nuda L)[J].Acta Agriculturae Boreali-Sinica,2006,21(4):31-34. |
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| Authors: | JIA Li-min FU Xiao-feng SUN Guo-qin SUN Rui-fen SI Qin Bateer LIU Li-ping LIU Jun-qing |
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| Institution: | 1. Biotechnology Centre, Inner Mongolia Academy of Agriculture and Animal Husbandry, Huhhot 010031, China;2. Sugarbeet Institute , Inner Mongolia Academy of Agriculture and Animal Husbandry, Huhhot 010031, China |
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| Abstract: | The influence of genotypes,medium and explant on induction,subculture and regeneration of oat callus have been studied by tissue culture of immature embryo and inflorescence of three oat varieties(line).The results showed that genetype and medium had an important role in callus induction.They were very significant and significant respectively on immature embryo and inflorescence.The formation of callus was also affected by explant and it was difference with the medium composition,inflorescence were easier to culture than immature embryo.2,4D concentration influenced callus growth and embryogenic callus generation and it can accelerate callus development and embryogenic callus generation in 3 mg/L. Callus from inflorescence of "Cao you no.1" had strong subculture capacity,it had 46.58% differentiation rate after 330 days subculture.This material had great potential in tissue culture and in genetic engineering. |
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| Keywords: | Avena nuda L Immature embryo Inflorescence Tissue culture |
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