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大丽轮枝菌拮抗细菌的分离筛选及Bv1-9菌株鉴定
引用本文:王全,王占利,李术娜,李红亚,朱宝成.大丽轮枝菌拮抗细菌的分离筛选及Bv1-9菌株鉴定[J].湖北农业科学,2009,48(5).
作者姓名:王全  王占利  李术娜  李红亚  朱宝成
作者单位:1. 河北农业大学生命科学学院,河北,保定,071001
2. 保定学院资源与环境系,河北,保定,071002
基金项目:河北省自然科学基金项目 
摘    要:对植物黄萎病病原菌大丽轮枝菌拮抗细菌进行了分离筛选及Bv1-9拮抗菌株鉴定.结果表明,从土壤中分离到891株细菌,初筛出具有拮抗活性的菌株83株,经过复筛选出一株具有较高拮抗活性的菌株Bv1-9,并对其进行了形态鉴定、生理生化特征鉴定和16S rDNA全序列分析,最终此菌株鉴定为花域芽孢杆菌,16S rDNA序列相似度达99.49%.

关 键 词:大丽轮枝菌  拮抗细菌  分离  鉴定  花域芽孢杆菌

Isolation of the Antagonistic Bacteria Against Verticillium dahliae and Identification of the Strain Bv1-9
WANG Quan,WANG Zhan-li,LI Shu-na,LI Hong-ya,ZHU Bao-cheng.Isolation of the Antagonistic Bacteria Against Verticillium dahliae and Identification of the Strain Bv1-9[J].Hubei Agricultural Sciences,2009,48(5).
Authors:WANG Quan  WANG Zhan-li  LI Shu-na  LI Hong-ya  ZHU Bao-cheng
Institution:1.College of Life Science;Agricultural University of Hebei Province;Baoding 071001;Hebei;China;2.Department of Resource and Environment;Baoding University;Baoding 071002;China
Abstract:Verticillium dahliae is a worldwide disastrous plant vascular disease infecting many important crops.Isolating and screening the antagonistic bacteria against Verticillium dahliae may contribute to the control of this disease.The strains hav-ing antagonistic activity against Verticillium dahliae were isolated and screened from the soil of cotton field from several dif-ferent regions.Totally 891 strains were obtained,of which 83 strains showed the antagonistic activities after preliminary screening.Strain Bv...
Keywords:Verticillium dahliae  antagonistic bacteria  isolation  identification  Bacillus vallismortis  
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