| 哈密瓜细菌性果斑病菌群体感应系统的检测 |
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| 引用本文： | 刘鹏,赵廷昌.哈密瓜细菌性果斑病菌群体感应系统的检测[J].植物保护,2009,35(6). |
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| 作者姓名： | 刘鹏 赵廷昌 |
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| 作者单位： | 中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京,100193 |
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| 基金项目： | 现代农业产业技术体系建设专项资金资助 |
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| 摘 要： | 本文利用群体感应信号报告菌Agrobacterium tumefaciens NTL4(pZLR4),在LB报告平板上对燕麦食酸菌西瓜亚种的19个菌株进行了初步检测,发现18个菌株有群体感应信号产生.用琼脂条法对菌株Pslb-94进一步检测,证实菌株Pslb-94存在群体感应系统.提取了该菌株信号物质,反相薄层层析(TLC)检测证明该菌株能产生群体感应信号分子.
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| 关 键 词： | 燕麦食酸菌西瓜亚种 群体感应 鉴定 |
Detection of the quorum-sensing of Acidovorax avenae subsp.citrulli |
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| Liu Peng,Zhao Tingchang.Detection of the quorum-sensing of Acidovorax avenae subsp.citrulli[J].Plant Protection,2009,35(6). |
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| Authors: | Liu Peng Zhao Tingchang |
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| Abstract: | As AHLs reporter strain,Agrobacterium tumefaciens NTIA(pZLR4)was used to detect the 19 strains of A.avenae subsp.citrulli,18 of which were found to produce signal molecules.Agar slice test was further used to detect the strain Pslb-94,and the results showed that it contained quorum-sensing system.The signal molecule was extracted and detected by TLC. |
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| Keywords: | Acidovorax avenae subsp citrulli quorum-sensing identification |
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