| 大孔树脂对刺葡萄籽中原花青素的纯化 |
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| 引用本文： | 刘东波 熊兴耀,王辉宪 马玉美罗启枚 谢 聃.大孔树脂对刺葡萄籽中原花青素的纯化[J].湖南农业大学学报(自然科学版),2010,36(1):39-44. |
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| 作者姓名： | 刘东波 熊兴耀 王辉宪 马玉美罗启枚 谢 聃 |
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| 作者单位： | 1. 湖南农业大学理学院,湖南,长沙,410128
2. 湖南农业大学园艺园林学院,湖南,长沙,410128 |
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| 基金项目： | 国家科技支撑计划项目 |
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| 摘 要： | 运用正交试验筛选树脂,探索原花青素在所选树脂上的吸附和解吸工艺条件.结果表明,所考察的YWD-06C、YWD-09F、YWD-03K7、YWD-04B1、HPD-300、HPD-700、D-101和DS-401树脂中,YWD-06C树脂为葡萄籽原花青素纯化的最佳树脂;最佳吸附条件:吸附温度20℃,吸附时间6h,溶液pH值为4;最佳解吸条件:解吸液为60%的乙醇水溶液,pH值为4,解吸时间6h,解吸温度25℃.通过动态试验,确定吸附速率为4B/h,洗脱速率为2BV/h,经该工艺所得纯化物中原花青素的含量达92.57%.
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| 关 键 词： | 刺葡萄籽 原花青素 大孔树脂 纯化 grape (Vitis davidii Fo?x) seeds |
| 收稿时间： | 2009/10/9 0:00:00 |
| 修稿时间： | 2009/10/9 0:00:00 |
Purification of proanthocyanidins from grape seeds by macroiporous adsorption resins |
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| LiuDongBo,Xiong Xingyao and Wang Huixian,MaYumei,LuoQimei,XieDan.Purification of proanthocyanidins from grape seeds by macroiporous adsorption resins[J].Journal of Hunan Agricultural University,2010,36(1):39-44. |
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| Authors: | LiuDongBo Xiong Xingyao and Wang Huixian MaYumei LuoQimei XieDan |
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| Institution: | Horticulture and Landscape College Hunan Agricultural University,College of Science Hunan agricultural university |
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| Abstract: | The technics of purification of proanthocyanidins from grape (Vitis davidii Fo?x) seeds were studied.Resin was chosen by orthogonal experiment,capability of adsorption and desorption and effect factors of proanthocyanidins on resins were reviewed,the best conditions for adsorption and desorption on the chosen resin of proanthocyanidins were researched.The result were as follows: the best chosen resin was YWD-06C,the optimal adsorption conditions were: temperature,20 ℃,adsorption time,6 h,pH,4;the optimal desorption conditions were: solvent 60% ethanol,pH,4,desorption time,6 h,temperature,25 ℃; the adsorption speed,4 BV/h and the desorption speed,2 BV/h,which were established by dynamic testing.The content of proanthocyanidins after purfication reached 92.57%. |
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| Keywords: | proanthocyanidins macroiporous adsorption resins purification |
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