| 梨20个品种S基因型的鉴定及新S-RNases基因克隆 |
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| 引用本文： | 衡伟,张绍铃,方成泉,吴华清,吴俊.梨20个品种S基因型的鉴定及新S-RNases基因克隆[J].园艺学报,2008,35(3):313-318. |
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| 作者姓名： | 衡伟 张绍铃 方成泉 吴华清 吴俊 |
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| 作者单位： | (南京农业大学园艺学院,南京 210095; 中国农业科学院果树研究所,辽宁兴城 125100) |
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| 基金项目： | 国家自然科学基金
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国家高技术研究发展计划(863计划) |
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| 摘 要： | 为了鉴定我国梨品种和一些野生类型个体的S基因型，应用S-RNase特异PCR扩增、克隆和测序，对其S-RNases基因核苷酸序列进行分析，鉴定了20个梨品种和野生类型个体的S基因型。起源于我国的'奥连'(SpS32)、'吊蛋'(SdSe)、'沙疙瘩'（S36Sd）品种和杏叶梨的一个类型（S22Sc）个体中存在西洋梨的S-RNase基因，证明S-RNase基因分化是在东方梨种群和西方梨种群的各个种形成之前。在秋子梨的'麦梨'、'内蒙古山梨'中发现了2个新S-RNases基因，命名为S40-、S41-RNase（DQ903313、DQ988687）。S40-和S41-RNase基因推导的部分氨基酸序列分别与苹果属S11-和S6-RNase的同源性为100%和94.4%，这表明S-RNase的存在可能在梨属和苹果属形成之前。
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| 关 键 词： | 梨 自交不亲和性 S基因型 S-RNase基因 |
| 收稿时间： | 2007-10-26 |
Identification of 20 S-genotypes and Cloning Novel S-RNases in Pyrus |
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| HENG Wei,ZHANG Shao-ling,FANG Cheng-quan,WU Hua-qing,WU Jun.Identification of 20 S-genotypes and Cloning Novel S-RNases in Pyrus[J].Acta Horticulturae Sinica,2008,35(3):313-318. |
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| Authors: | HENG Wei ZHANG Shao-ling FANG Cheng-quan WU Hua-qing WU Jun |
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| Institution: | ( College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China; Fruit Research Institute of Chinese Academy of Agricultural Sciences, Xingcheng, Liaoning 125100, China) |
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| Abstract: | To identify S-genotypes of pear cultivars or a few types of wild species native to China，S-RNase specific PCRs were used. After cloning and sequencing of PCR products , the S-genotypes of 20 pear cultivars or wild species individuals were determined. Subsequent sequence analysis showed that 'Aolian' (SpS32) from Pyrus. sinkiangensis Yü, 'Diaodan'(SdSe) from P. phaeocarpa Rehd, 'Shageda'(S36Sd) from P. xerophila Yü and one type of Xingyeli(S22Sc) originated from China shared some S-RNases with species from P. communis , which provided with evidence that S-RNase alleles occurred before the divergence of oriental and occidental pear. Two novel S-RNases were also discovered in 'Maili' and 'Neimenggushanli' in P. ussuriensis, and deposited as S40-, S41-RNase under the accession numbers of DQ903313 and DQ988687, respectively, whose deduced amino acid sequences showed high similarities to S11-RNase(100% ) and S6-RNase (94.4%) in Malus, respectively. The high similar score between S-RNase in Pyrus and Malus indicated that S-RNase existence predated the speciation between Pyrus and Malus. |
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| Keywords: | Pyrus Pyrus Self-incompatibility S-genotype S-RNase gene S-RNase gene |
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