| 应用MNP-RT-PCR方法检测黄瓜绿斑驳花叶病毒 |
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| 引用本文： | DENG Cong-liang,江明,WANG Wan-chun,聂棱,LIANG Xin-miao,杨小霞,GAO Wen-na,吕玉峰.应用MNP-RT-PCR方法检测黄瓜绿斑驳花叶病毒[J].植物病理学报,2008,38(4):436-440. |
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| 作者姓名： | DENG Cong-liang 江明 WANG Wan-chun 聂棱 LIANG Xin-miao 杨小霞 GAO Wen-na 吕玉峰 |
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| 作者单位： | 1. 北京出入境检验检疫局植物检疫中心, 北京 100026;2. 北京金纳信生物科技有限公司, 北京 100081 |
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| 基金项目： | 国家质量监督检验检疫总局资助项目 |
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| 摘 要： | A novel RT-PCR method integrated with Magnetic Nano Particles (MNP), MNP-RT-PCR, was set up for detection of Cucumber green mottle mosaic virus (CGMMV). After the virus particles in crude sap were concentrated by MNP, viral RNAs were released and were detected by RT-PCR. CGMMV could be detected in as less as 10 ng watermelon leaf materials. Compared with normal RT-PCR, the method decreased the inhibitors of plant material and steps for extracting RNA, and also increased the sensitivity of RT-PCR detection in less time. The method is simple and suitable for quick detection of plant virus in a large number of samples.
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| 关 键 词： | 烟草花叶病毒 检测灵敏度 PCR方法 技术应用 黄瓜 RT MNP 葫芦科植物 |
Detection of Cucumber green mottle mosaic virus using MNP-RT-PCR method |
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| DENG Cong-liang,JIANG Ming,WANG Wan-chun,NIE Leng,LIANG Xin-miao,YANG Xiao-xia,GAO Wen-na,LU Yu-feng.Detection of Cucumber green mottle mosaic virus using MNP-RT-PCR method[J].Acta Phytopathologica Sinica,2008,38(4):436-440. |
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| Authors: | DENG Cong-liang JIANG Ming WANG Wan-chun NIE Leng LIANG Xin-miao YANG Xiao-xia GAO Wen-na LU Yu-feng |
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| Institution: | 1. Beijing Entry-exit Inspection and Quarantine Bureau, Beijing 100026, China;2. Kingnaing Biotech Co., Ltd., Beijing 100081, China |
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| Abstract: | A novel RT-PCR method integrated with Magnetic Nano Particles (MNP), MNP-RT-PCR, was set up for detection of Cucumber green mottle mosaic virus (CGMMV). After the virus particles in crude sap were concentrated by MNP, viral RNAs were released and were detected by RT-PCR. CGMMV could be detected in as less as 10 ng watermelon leaf materials. Compared with normal RT-PCR, the method decreased the inhibitors of plant material and steps for extracting RNA, and also increased the sensitivity of RT-PCR detection in less time. The method is simple and suitable for quick detection of plant virus in a large number of samples. |
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| Keywords: | Magnetic Nano Particles (MNP) Cucumber green mottle mosaic virus RT-PCR |
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