| 松花菜花球腐烂病病原分离鉴定及室内药剂筛选 |
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| 引用本文： | 王国荣,刘晓曦,吴金丹,王文凤,苏珍珠,楼兵干.松花菜花球腐烂病病原分离鉴定及室内药剂筛选[J].植物保护,2015,41(6):67-71. |
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| 作者姓名： | 王国荣 刘晓曦 吴金丹 王文凤 苏珍珠 楼兵干 |
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| 作者单位： | 1. 浙江省杭州市萧山区农业技术推广中心，杭州 311203；2.浙江大学生物技术研究所，杭州 310029；3.安徽农业大学植物保护学院，合肥 230036 |
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| 基金项目： | 浙江省“三农六方”资助项目(N20120623) |
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| 摘 要： | 松花菜花球腐烂病是近年来发生在浙江省松花菜种植区的一种严重病害,主要危害松花菜花球、花梗,引起花球变褐腐烂,花梗发黑枯萎,造成严重经济损失。通过对松花菜花球腐烂病典型症状样本的采集,病原菌的分离、纯化,致病性测定,形态观察,16SrDNA序列分析,脂肪酸分析和Biolog鉴定,明确病原菌为野油菜黄单胞菌野油菜致病变种Xanthomonas campestris pv.campestris(Pammel)Dye。选择8种杀菌剂采用K-B灭菌滤纸片法对病菌进行室内抑菌测定,结果表明,72%农用硫酸链霉素和PHMB对该病菌有较强的抑制作用,其次中生菌素和三氯异氰尿酸也有一定的抑制作用。
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| 关 键 词： | 松花菜花球腐烂病 杀菌剂 PHMB 药剂筛选 |
| 收稿时间： | 2014/12/9 0:00:00 |
| 修稿时间： | 2/2/2015 12:00:00 AM |
Isolation, identification and fungicide screening of the pathogen of broccoli flower-ball rot disease in laboratory |
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| Wang Guorong,Liu Xiaoxi,Wu Jindan,Wang Wenfeng,Su Zhenzhu,Lou Binggan.Isolation, identification and fungicide screening of the pathogen of broccoli flower-ball rot disease in laboratory[J].Plant Protection,2015,41(6):67-71. |
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| Authors: | Wang Guorong Liu Xiaoxi Wu Jindan Wang Wenfeng Su Zhenzhu Lou Binggan |
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| Abstract: | Broccoli flower ball rot disease has become a serious disease in recent years in broccoli producing areas of Zhejiang Province. It mainly infects the flower ball and stalks of broccoli, causing the flower ball brown and rot, and the stalks black and withered, and finally leads to a serious economic loss. In this study, we collected samples with typical symptoms of this disease, isolated and purified the pathogen, and then conducted a series of experiments, including the pathogenicity determination, observation on morphological characteristics, analysis of 16S rDNA gene sequences and fatty acid methyl esters (FAME), and Biolog identification. All of these results showed that the pathogen was Xanthomonas campestris pv. campestris (Pammel) Dye. Moreover, we selected 8 kinds of fungicides to do the bacterial inhibition assay in the laboratory with K-B sterile filter paper. The results showed that 72% agricultural streptomycin sulfate and PHMB had strong inhibition action to the pathogen, and zhongshengmycin and trichloroiso cyanuric acid also had inhibition action to the pathogen to a certain extent. |
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| Keywords: | broccoli flower-ball rot disease fungicide PHMB fungicide screening |
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