| 甘蓝型黄籽油菜子叶离体培养初报 |
| |
| 引用本文： | 程利华,殷家明,林呐,周清元,唐章林,李加纳.甘蓝型黄籽油菜子叶离体培养初报[J].中国农学通报,2005,21(7):31-31. |
| |
| 作者姓名： | 程利华 殷家明 林呐 周清元 唐章林 李加纳 |
| |
| 作者单位： | 西南农业大学农学与生命科学学院重庆市油菜工程技术研究中心,重庆,400716 |
| |
| 基金项目： | 国家863计划项目"黄籽油菜新品种选育"(2002AA207009),"优质高效甘蓝型黄籽油菜新品种、新材料选育"(2004AA241100)和948项目"油菜特异资源和育种新技术的引进、改良及创新研究"(2003-Q04)资助. |
| |
| 摘 要： | 以甘蓝型油菜黄籽双单倍体品系04K91为供试材料,以无菌苗子叶为离体培养外植体,考察了苗龄（7d、10d、13d）与外植体部位及大小（带柄子叶、1/4子叶片、1/8子叶片和子叶柄切段）对芽再生频率的影响。方差分析结果表明,苗龄对芽再生频率有显著影响,外植体对芽再生频率有极显著影响,而苗龄×外植体对芽再生频率影响不显著。多重比较结果表明,以7d苗龄无菌苗的带柄子叶或1/4子叶片为外植体较好,出芽率较高,达90%以上。
|
| 关 键 词： | 甘蓝型油菜 黄籽 子叶 离体培养 |
Preliminary Research on in vitro Culture of Cotyledon from Yellow-seeded Brassica napus L. |
| |
| Cheng Lihu,Yin Jiaming,Lin N,Zhou Qingyuan,Tang Zhanglin,Li Jiana.Preliminary Research on in vitro Culture of Cotyledon from Yellow-seeded Brassica napus L.[J].Chinese Agricultural Science Bulletin,2005,21(7):31-31. |
| |
| Authors: | Cheng Lihu Yin Jiaming Lin N Zhou Qingyuan Tang Zhanglin Li Jiana |
| |
| Institution: | (Chongqing Rapeseed Engineering & Technology Research Center of College of Agronomy and Life Science, Southwest Agricultural University, Chongqing 400716) |
| |
| Abstract: | The cotyledon with petiole, 1/4 piece of cotyledonary laminae, 1/8 piece of cotyledonary laminae and segment of cotyledonary petiole from 7, 10 and 13 days old aseptic seedling of the yellow-seeded Brassica napus L. double haploid line 04K91 were cultured in vitro. The effect of seedling age and explant type on shoot regeneration frequency was explored. The ana1ysis of variance showed that seedling age had significant influence, explant type had greatly significant influence,and that seedling age×explant type had no remarkable effect on shoot regeneration frequency. Multiple comparisons suggested that cotyledon with petiole or 1/4 piece of cotyledonary laminae from 7 days old aseptic seedling had much higher shoot regeneration frequency, reaching to over 90% . |
| |
| Keywords: | Brassica napus L yellow-seeded cotyledon In vitro culture |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国农学通报》浏览原始摘要信息 |
| 点击此处可从《中国农学通报》下载免费的PDF全文 |
|
