| 大鳍鳠免疫球蛋白M 重链基因的克隆及表达分析 |
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| 引用本文： | 李春涛,张其中,杨莹莹,朱成科,李超,陈霞.大鳍鳠免疫球蛋白M 重链基因的克隆及表达分析[J].水产学报,2011,35(11):1684-1693. |
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| 作者姓名： | 李春涛 张其中 杨莹莹 朱成科 李超 陈霞 |
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| 作者单位： | 1. 西南大学生命科学学院,水产科学重庆市市级重点实验室,三峡库区生态环境教育部重点实验室,淡水生物生殖与发育教育部重点实验室,重庆400715
2. 暨南大学水生生物研究所,广东广州,510632 |
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| 基金项目： | 国家“八六三”高技术研究发展计划(2011AA10A216);重庆市科技攻关计划项目(CSTC.1010AC1116);三峡库区生态环境教育部重点实验室基金资助项目 |
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| 摘 要： | 用RACE-PCR和RT-PCR方法获得大鳍鳠分泌型免疫球蛋白M(sIgM)重链基因的全长cDNA序列.大鳍鳠sIgM的cDNA全长为1 992 bp,包含5 '非编码区53 bp,3’非编码区226 bp,开放阅读框1 713,编码570个氨基酸.推测的大鳍鳠sIgM蛋白质序列可变区含有4个骨架区(FR)和3个互补决定区(CDR),恒定区(CH)含有CH1、CH2、CH3和CH4 4个部分.与其它6种硬骨鱼类IgM氨基酸序列比对分析表明,大鳍鳠sIgM存在半胱氨酸和色氨酸保守位点；其氨基酸序列与斑点叉尾鲴IgM的相似性最高,为54.3％；与石斑鱼的相似性最低,为24.6％.进化树分析表明,大鳍鳠sIgM与斑点叉尾鲴聚为一支.RT-PCR显示,大鳍鳠IgM基因主要在血细胞、脾脏、头肾和中肾中转录表达；注射嗜水气单胞菌后,血细胞、脾脏和头肾IgM重链基因转录表达量在1～10 d内有显著上升.
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| 关 键 词： | 大鳍鳠 IgM重链基因cDNA 克隆 实时荧光定量PCR 组织分布 |
| 收稿时间： | 6/3/2011 12:00:00 AM |
| 修稿时间： | 2011/7/10 0:00:00 |
Molecular cloning and expression analysis of the IgM heavy chain gene in largefin longbarbel catfish(Mystus macropterus) |
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| LI Chun tao,ZHANG Qi zhong,YANG Ying ying,ZHU Cheng ke,LI Chao and CHEN Xia.Molecular cloning and expression analysis of the IgM heavy chain gene in largefin longbarbel catfish(Mystus macropterus)[J].Journal of Fisheries of China,2011,35(11):1684-1693. |
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| Authors: | LI Chun tao ZHANG Qi zhong YANG Ying ying ZHU Cheng ke LI Chao and CHEN Xia |
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| Institution: | Key Laboratory of Aquatic Science of Chongqing,Key laboratory Eco-environments in Three Gorges Reservoir Region Ministry of Education,Key Laboratory of Freshwater Fish Reproduction and Development Ministry of Education,School of Life Science,Southwest University,Hydrobiology Institute of Jinan University,Key Laboratory of Aquatic Science of Chongqing,Key laboratory Eco-environments in Three Gorges Reservoir Region Ministry of Education,Key Laboratory of Freshwater Fish Reproduction and Development Ministry of Education,School of Life Science,Southwest University,Key Laboratory of Aquatic Science of Chongqing, Key laboratory Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), School of Life Science, Southwest University,Key Laboratory of Aquatic Science of Chongqing,Key laboratory Eco-environments in Three Gorges Reservoir Region Ministry of Education,Key Laboratory of Freshwater Fish Reproduction and Development Ministry of Education,School of Life Science,Southwest University,Key Laboratory of Aquatic Science of Chongqing,Key laboratory Eco-environments in Three Gorges Reservoir Region Ministry of Education,Key Laboratory of Freshwater Fish Reproduction and Development Ministry of Education,School of Life Science,Southwest University |
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| Abstract: | |
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| Keywords: | Mystus macropterus cDNA of immunoglobulin M heavy-chain gene clone real-time PCR tissue distribution |
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