| 陕西省部分地区猪瘟流行毒株与疫苗毒株E2基因主要抗原区序列变异分析 |
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| 引用本文： | 朱小甫,吴旭锦.陕西省部分地区猪瘟流行毒株与疫苗毒株E2基因主要抗原区序列变异分析[J].上海交通大学学报(农业科学版),2011,29(6):24-28. |
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| 作者姓名： | 朱小甫 吴旭锦 |
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| 作者单位： | 咸阳职业技术学院生物科技系,动物疫病分子生物学诊断实验室,陕西咸阳712000 |
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| 基金项目： | 咸阳市科技计划项目(211K04-12) |
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| 摘 要： | 研究陕西省猪瘟病毒E2基因主要抗原区变异情况,为猪瘟防控提供依据.用巢式PCR方法对猪瘟疫苗和采自陕西省部分地区的疑似猪瘟病料进行E2基因主要抗原区扩增并测序,将测序结果与HCLV疫苗株和Shimen株E2基因进行序列比对分析.结果表明,23株流行毒株之间的核苷酸同源性在86.0％～100％,氨基酸同源性在87.8％～...
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| 关 键 词： | 猪瘟 E2基因 序列分析 |
Sequence Analysis of Main Antigen Variation of E_2 Gene of Vaccine Strains andVirulent Classical Swine Fever Virus Isolated in Shanxi Province |
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| ZHU Xiao-fu,WU Xu-jin.Sequence Analysis of Main Antigen Variation of E_2 Gene of Vaccine Strains andVirulent Classical Swine Fever Virus Isolated in Shanxi Province[J].Journal of Shanghai Jiaotong University (Agricultural Science),2011,29(6):24-28. |
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| Authors: | ZHU Xiao-fu WU Xu-jin |
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| Institution: | ZHU Xiao-fu,WU Xu-jin (Animal Epidemic Disease Diagnostic Laboratory of Molecular Biology,Department of Biology Science & Technology,Xianyang Vocational Technical College,Xianyang 712000,China) |
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| Abstract: | To get useful data for the prevention and control of classical swine fever,the main antigen variation of E2 gene of classical swine fever virus in Shaanxi Province were analyzed by nested RT-PCR amplified.The main antigen of E2 gene of the vaccines and parts of samples from the suspected classical swine fever from Shaanxi Province was sequenced,which was compared with HCLV vaccine strains and Shimen strain E2 gene sequence.The results showed the nucleotide and amino acid homology of 23 pandemic strains are ... |
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| Keywords: | classical swine fever E2 gene cloning sequence analysis |
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