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| 矾根的组织培养与快速繁殖 | |
| 引用本文： | 陈宏,唐莹,施月欢,吴杰.矾根的组织培养与快速繁殖[J].上海农业学报,2011,27(4). |
| 作者姓名： | 陈宏 唐莹 施月欢 吴杰 |
| 作者单位： | 1. 上海精文绿化艺术发展有限公司,上海,200052 2. 上海市宝山区农业技术推广中心,上海,201901 3. 上海市宝山区生物技术中心,上海,201908 |
| 摘 要： | 以矾根带茎尖幼嫩茎段为外植体,以MS为基本培养基,研究了不同激素配比条件下矾根的组织培养与快速繁殖技术。结果表明:适宜的诱导增殖培养基为MS+6-BA 0.5 mg/L+NAA 0.05mg/L;继代培养基为MS+6-BA 0.1mg/L+NAA 0.01mg/L;生根培养基为1/2 MS+NAA 0.2mg/L+AC 1.0g/k。生根苗移入泥炭土:珍珠岩=2:1的基质中,成活率达80%以上。 |
| 关 键 词： | 矾根 组织培养 快速繁殖 |
Tissue culture and rapid propagation of Heuchera micrantha |
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| CHEN Hong,TANG Ying,SHI Yue-huan,WU Jie.Tissue culture and rapid propagation of Heuchera micrantha[J].Acta Agriculturae Shanghai,2011,27(4). | |
| Authors: | CHEN Hong TANG Ying SHI Yue-huan WU Jie |
| Institution: | CHEN Hong~1,TANG Ying~1,SHI Yue-huan~(2*),WU Jie~3 (1 Shanghai Jingwen Greening & Arts Development Co.,Ltd,Shanghai 200052,China,2 Baoshan District Agro-Technology Extension Center,Shanghai 201901,3 Biotechnology Center of Baoshan District,Shanghai 201908,China) |
| Abstract: | The young stem segments with stem apexes were used as explants.and MS as minimal medium to study the technique of tissue culture and rapid propagation of H.micrantha under different hormone concentrations.The results showed that the appropriate media were MS+0.5 mg/L 6-BA+ 0.05 mg/L NAA for induction and proliferation.MS+0.1 mg/L 6-BA+0.01 mg/L NAA for subculture, and 1/2 MS+0.2 mg/L NAA+1.0 g/L AC for rooting.The rooted plantlets were transplanted into the substrate(peat soil:perlite=2:1) and their surviva... |
| Keywords: | Heuchera micrantha Tissue culture Rapid propagation |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |