|
|||||
|
|
| 上海地区猪戊型肝炎病毒部分序列的克隆与分析 | |
| 引用本文： | 宁海强,李震,于瑞嵩,张佩华,牛钟相.上海地区猪戊型肝炎病毒部分序列的克隆与分析[J].西南农业学报,2007,20(5):1105-1108. |
| 作者姓名： | 宁海强 李震 于瑞嵩 张佩华 牛钟相 |
| 作者单位： | 1. 山东农业大学,动物科技学院,山东,泰安,271018 2. 上海市农业科学院,畜牧兽医研究所,上海,201106;上海市农业遗传育种重点实验室,上海,201106 3. 上海市农业科学院,畜牧兽医研究所,上海,201106 |
| 基金项目： | 上海市农业科学院青年科技基金 |
| 摘 要： | 对上海地区几个猪场的戊型肝炎感染情况进行了调查,共采集粪便样品36份,通过RT-nPCR的方法应用通用引物对病毒基因组的ORF2部分片断进行扩增,36份样品中有5份RT-nPCR阳性,HEV RNA阳性率为13.9%,去除相同的序列,得到2株HEV的ORF2部分序列。序列分析表明,这两段序列与基因I、II、III、IV的遗传距离分别为0.183、0.207、0.230、0.102和0.177、0.226、0.240、0.108。系统进化分析显示,这两株病毒都属于基因Ⅳ型。 |
| 关 键 词： | 猪 戊型肝炎病毒 RT-nPCR 序列分析 基因型 |
| 文章编号： | 1001-4829（2007）05-1105-04 |
| 修稿时间： | 2006-11-07 |
Partial cloning and analysis of swine HEV isolates in Shanghai |
|
| NING Hai-qiang,LI Zhen,YU Rui-song,ZHANG Pei-hua,NIU Zhong-xiang.Partial cloning and analysis of swine HEV isolates in Shanghai[J].Southwest China Journal of Agricultural Sciences,2007,20(5):1105-1108. | |
| Authors: | NING Hai-qiang LI Zhen YU Rui-song ZHANG Pei-hua NIU Zhong-xiang |
| Abstract: | This study was to determine the condition of prevalence at several pig farms in Shanghai.We collected 36 swine fecal samples.Through the RT-PCR method,we amplified the partial ORF2 of HEV genome using univerisal primers.5 samples of 36 were positive for RT-nPCR,and the ratio of positive for RT-nPCR was 13.9 %.Taking out the same sequence,we got two partial sequences of HEV ORF2.The analysis of sequence suggests that the genetic distance between the two sequences and genotype I,II,III,IV were 0.183,0.207,0.230,0.102 and 0.177,0.226,0.240,0.108,respectively.Phylogenetic analysis indicated that both sequences belonged to genotype IV. |
| Keywords: | pig hepatitis E virus RT-nPCR sequence analysis genotype |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |