| 加味丹参饮预处理调节ODC活性抗大鼠心肌缺血再灌注损伤研究 |
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| 作者姓名： | 任婷 杨胜辉 饶春梅 彭霞 孙彦波 吴若霞 陈聪 成细华 黄政德 |
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| 作者单位： | 湖南中医药大学, 湖南 长沙 410208,湖南中医药大学, 湖南 长沙 410208,湖南中医药大学, 湖南 长沙 410208,湖南中医药大学, 湖南 长沙 410208,湖南中医药大学, 湖南 长沙 410208,湖南中医药大学, 湖南 长沙 410208,湖南中医药大学, 湖南 长沙 410208,湖南中医药大学, 湖南 长沙 410208,湖南中医药大学, 湖南 长沙 410208 |
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| 基金项目： | 国家自然科学基金资助项目（81373576，81503536）；湖南省教育厅项目（14B136，14C0872）；湖南省中医药管理局重点课题（201304）；湖南省科技厅一般项目（2014SK3034）；中西医结合防治心脑疾病的相关基础研究湖南省高校科技创新团队科研项目（5）。 |
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| 摘 要： | 目的 探讨加味丹参饮通过调节鸟氨酸脱羧酶（ODC）活性在抗SD大鼠心肌缺血再灌注损伤（IRI）中的保护机制。方法 建立SD大鼠心肌IRI模型，设对照组、假手术组、缺血再灌注损伤（IRI）组、加味丹参饮（JWDSY）+IRI组共4组。酶联免疫（ELISA）法测定ODC含量，实时荧光定量PCR（RT-PCR）方法检测ODC mRNA表达水平，免疫印迹（Western blot）法检测ODC蛋白表达水平，高效液相色谱检测心肌组织中多胺的含量。结果 与对照组比较，假手术组的ODC含量、ODC mRNA及蛋白表达水平均无统计学意义（P>0.05）；与假手术组比较，IRI组的ODC含量显著增加、ODC mRNA及蛋白表达水平显著升高，差异有统计学意义（P<0.01）；与IRI组比较，JWDSY+IRI组ODC含量显著减少，ODC mRNA和蛋白表达水平显著降低，差异有统计学意义（P<0.01）。结论 加味丹参饮可能通过调节ODC活性，从而发挥对IRI后心肌的保护作用。
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| 关 键 词： | 加味丹参饮 心肌 缺血再灌注损伤 多胺 鸟氨酸脱羧酶 |
| 收稿时间： | 2016/8/13 0:00:00 |
Study of Modified Danshen Decoction Pretreatment on Adjusting ODC Activity Against the Rats Myocardial Ischemia Reperfusion Injury |
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| Authors: | REN Ting YANG Shenghui RAO Chunmei PENG Xi SUN Yanbo WU Ruoxi CHEN Cong CHENG Xihua and HUANG Zhengde |
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| Institution: | Hunan University of Chinese Medicine, Changsha, Hunan 410208, China,Hunan University of Chinese Medicine, Changsha, Hunan 410208, China,Hunan University of Chinese Medicine, Changsha, Hunan 410208, China,Hunan University of Chinese Medicine, Changsha, Hunan 410208, China,Hunan University of Chinese Medicine, Changsha, Hunan 410208, China,Hunan University of Chinese Medicine, Changsha, Hunan 410208, China,Hunan University of Chinese Medicine, Changsha, Hunan 410208, China,Hunan University of Chinese Medicine, Changsha, Hunan 410208, China and Hunan University of Chinese Medicine, Changsha, Hunan 410208, China |
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| Abstract: | Objective To explore the protective mechanism of modified Danshen decoction on SD rats myocardial ischemia-reperfusion injury (IRI) by ornithine decarboxylase (ODC) activity. Methods The SD rat models of myocardial IRI were established, the four groups of control group, sham-operation group, ischemia/reperfusion (IRI) group, Jiawei Danshen Decoction (JWDSY) + IRI group were assigned. The content of ODC was determined by ELISA method. The ODC mRNA expression level was detected by fluorescence quantitative RT-PCR method. The ODC protein expression level was detected by Western blot method, and the polyamine content was determined by HPLC. Results Compared with the control group, the content of ODC, ODC mRNA and protein expression level in sham-operationa group were increased, the difference had no statistically significant (P>0.05), while compared with the sham-operation group, the content of ODC, ODC mRNA and protein expression level in IRI group were increased significantlay (P<0.01). Compared with IRI group, the ODC content in JWDSY + IRI group was significantly reduced, and the ODC mRNA and protein expression level were decreased significantly, the difference was statistically significant (P<0.01). Conclusion Modified Danshen decoction could adjust the ODC activity, and then play a protective effect on myocardium after IRI. |
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| Keywords: | modified Danshen decoction cardiac muscle ischemia reperfusion injury polyamine orinithine decarboxylase |
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