| 柠檬酸对小鼠睾丸组织抗氧化酶活性的影响 |
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| 引用本文： | 张鹏飞,吕琼霞,刘玉梅,邓雯,陈晓光,王艳杰.柠檬酸对小鼠睾丸组织抗氧化酶活性的影响[J].动物医学进展,2012,33(8):115-118. |
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| 作者姓名： | 张鹏飞 吕琼霞 刘玉梅 邓雯 陈晓光 王艳杰 |
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| 作者单位： | 河南科技大学动物科技学院,河南洛阳,471003 |
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| 基金项目： | 河南科技大学人才基金项目，河南科技大学科研基金项目 |
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| 摘 要： | 为研究柠檬酸对小鼠睾丸组织抗氧化酶活性的影响,将40只健康昆明系小鼠随机分为对照组(生理盐水)、柠檬酸低(180mg/kg)、中(270mg/kg)、高(360mg/kg)剂量组,各组小鼠每周腹腔注射相应剂量试剂0.2mL,连续注射3周,第3次注射后的第7天时颈椎脱臼处死小鼠,取其睾丸组织制作匀浆液检测过氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、乳酸脱氢酶(LDH)、总一氧化氮合酶(T-NOS)活性及丙二醛(MDA)含量。结果表明,与对照组相比,中、高柠檬酸剂量组小鼠睾丸组织SOD活性显著降低(P<0.05);柠檬酸处理组GSH-Px活性、MDA含量均显著下降(P<0.05);随着柠檬酸剂量的增加,LDH、NOS活性分别呈下降、上升趋势。此结果提示,一定剂量的柠檬酸可以降低小鼠睾丸组织SOD、GSH-Px活性,但可能通过NO途径减少睾丸细胞膜脂质过氧化,提高细胞膜的稳定性。
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| 关 键 词： | 柠檬酸 小鼠 睾丸 超氧化物歧化酶 谷胱甘肽过氧化物酶 总一氧化氮合成酶 |
Effects of Citric Acid on Activities of Antioxidant Enzymes of Testis in Mice |
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| ZHANG Peng-fei
,
L Qiong-xia
,
LIU Yu-mei
,
DENG Wen
,
CHEN Xiao-guang
,
WANG Yan-jie.Effects of Citric Acid on Activities of Antioxidant Enzymes of Testis in Mice[J].Progress In Veterinary Medicine,2012,33(8):115-118. |
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| Authors: | ZHANG Peng-fei L Qiong-xia LIU Yu-mei DENG Wen CHEN Xiao-guang WANG Yan-jie |
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| Institution: | ZHANG Peng-fei
,
L(U) Qiong-xia
,
LIU Yu-mei
,
DENG Wen
,
CHEN Xiao-guang
,
WANG Yan-jie |
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| Abstract: | To study effects of citric acid on the activities of antioxidant enzymes of testis in mice,40 healthy Kunming mice were randomly divided into control group(0.9% saline),low dose group(180 mg/kg),middle dose group(270 mg/kg) and high dose group(360 mg/kg).All the mice were received an injection of 0.2 mL corresponding reagent each week for 3 weeks.After the third injection of seventh day,all the mice were killed by cervical dislocation,superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),lactate dehydrogenase(LDH),total nitric oxide synthase(T-NOS) activities and malondialdehyde(MDA) contents were detected with the testicular tissue homogenate.The results showed that the SOD activity was significantly decreased in middle and high dose groups(P<0.05) compared with the control group,the activity of GSH-Px and MDA content were significantly degraded(P<0.05) in the citric acid treatment groups,with citric acid dose increased,the LDH and NOS activities were showed decreasing,rising trend respectively.The results suggested that a certain dose of citric acid can degrade the SOD,GSH-Px activities of testis in mice,but the reduction of lipid peroxidation and the stability in testicular cell membrane may be via NO pathway. |
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| Keywords: | citric acid mouse testis superoxide dismutase glutathione peroxidase total nitric oxide synthase |
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