| 以ITS-1+序列鉴定牛源环孢子虫 |
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| 引用本文： | 肖淑敏,李国清,周荣琼,李韦华.以ITS-1+序列鉴定牛源环孢子虫[J].中国兽医学报,2007,27(1):59-61,65. |
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| 作者姓名： | 肖淑敏 李国清 周荣琼 李韦华 |
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| 作者单位： | 华南农业大学,兽医学院,广东,广州,510642 |
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| 基金项目： | 国家自然科学基金;广东省自然科学基金 |
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| 摘 要： | 根据OenBank上已发表的环孢子虫（Cyclospora）rDNA序列设计并合成1对引物，利用PCR技术时首次在牛体内发现的形态学特征与人环孢子虫（Cyclospora cayetanensis）极为相似的牛源环孢子虫的ITS-1＋序列进行了扩增。将PCR扩增出的片段纯化后，克隆至pGEM—TEasy载体后进行序列测定，并利用NCBI在线BLAST程序对测序结果进行了同源性比较和序列分析。分析结果显示，扩增的ITS-1＋大小为865bp的片段，包含18S部分序列（371bp）、ITS-1全序列（385bp）和5．8S部分序列（109bp）。序列同源性分析表明，该牛源环抱子虫为艾美尔科原虫，但不同于目前已知的各种艾美尔科原虫，可能是一种新发现的原虫。
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| 关 键 词： | 牛源环孢子虫 分子鉴定 |
| 文章编号： | 1005-4545（2007）01-0059-03 |
| 修稿时间： | 2005-05-28 |
Molecular identification of Cyclospora-like species from dairy cattle based on ITS-1+ region |
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| XIAO Shu-min,LI Guo-qing,ZHOU Rong-qiong,LI Wei-hua.Molecular identification of Cyclospora-like species from dairy cattle based on ITS-1+ region[J].Chinese Journal of Veterinary Science,2007,27(1):59-61,65. |
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| Authors: | XIAO Shu-min LI Guo-qing ZHOU Rong-qiong LI Wei-hua |
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| Institution: | College of Veterinary Medicine, South China Agricultural University ,Guangzhou 510642,China |
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| Abstract: | According to the published sequence of the rDNA gene of Cyclospora in GenBank,a pair of primers was designed to amplify the ITS-1 region of the Cyclospora-like species from dairy cattle by PCR.The amplicons was cloned into pGEM-T Easy vector.The insert was successfully sequenced,and the result revealed that the insert was 856 bp in length and consisted of partial 18S,5.8S and complete ITS-1 DNA sequences.The dairy cattle-associated Cyclospora-like species was identified as a protozoa which belongs to Eimeriidae based on the partial 18S and 5.8S DNA sequences. It may be a new species since it has a specific ITS-1 sequence. |
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| Keywords: | ITS-1 PCR |
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