| 反义抑制ghrelin对VEGF及其受体Fit-1 mRNA表达的影响 |
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| 引用本文： | 姜丽萍,;祝啸先,;游存厚,;乌日古木拉,;王芳,;张文娟,;刘德斌,;杜晨光,;李海军,;包福祥,;赵鹏伟,;鲍庆江,;曹贵方.反义抑制ghrelin对VEGF及其受体Fit-1 mRNA表达的影响[J].安徽农业科学,2009(33):16277-16279. |
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| 作者姓名： | 姜丽萍 ;祝啸先 ;游存厚 ;乌日古木拉 ;王芳 ;张文娟 ;刘德斌 ;杜晨光 ;李海军 ;包福祥 ;赵鹏伟 ;鲍庆江 ;曹贵方 |
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| 作者单位： | [1]内蒙古农业大学动物科学与医学学院,内蒙古呼和浩特010018; [2]内蒙古医学院,内蒙古呼和浩特010059; [3]内蒙古自治区医院,内蒙古呼和告特010017; [4]内蒙古妇幼保健院,内蒙古呼和浩特010020; [5]内蒙古自治区人民检察院技术处,内蒙古呼和浩特010011 |
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| 基金项目： | 国家自然科学基金项目（30860201）. |
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| 摘 要： | ［目的］ 探讨ghrelin对蒙古绵羊脐静脉内皮细胞的血管内皮生长因子VEGF及其受体Flt-1 mRNA表达的影响。［方法］ 试验分为4组：Ⅰ组，空白对照组；Ⅱ组，脂质体组；Ⅲ组（SCON），20 μmol/L正义寡核苷酸组；Ⅳ组（ASCON），20 μmol/L反义寡核苷酸组。上述各组均在培养24、36、48 h后，采用实时荧光定量方法检测VEGF及其受体Flt-1 mRNA表达情况的变化。［结果］VEGF mRNA在Ⅰ、Ⅱ组表达量无差异，具有较高的表达量，随着时间的延长无明显变化；在Ⅲ组VEGF mRNA 表达略有降低，但与Ⅰ和Ⅱ组间无显著性差异（P＞0.05）；Ⅳ组（反义寡核苷酸组）的VEGF mRNA表达量显著下降（P＜0.05），并且随着时间延长逐渐下降。VEGF受体FLT-1 mRNA的表达与VEGF相似。［结论］反义抑制ghrelin对VEGF及其受体Flt-1的mRNA表达有下调作用。
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| 关 键 词： | 生长素 血管内皮生长因子 受体 寡核苷酸类 反义 |
Effects of Ghrelin Antisense Inhibition on VEGF and Its Receptor Fit-1 mRNA Expression |
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| Institution: | JIANG Li-ping et al ( College of Animal Science and Animal Medicine, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018) |
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| Abstract: | Objective ] This study was to investigate the effect of VEGF and its receptor Fh-1 mRNA expression in Mongolia sheep＇ s umbilical vein endothelial cells by ghrelin antisense inhibition. Method] Experiments were divided into 4 groups：group Ⅰ （blank control group） ; group Ⅱ （liposome group） ; group Ⅲ（SCON group： 20 μmol/L sense oligonucleotide） ; group Ⅳ （ASCON： 20 μmol/L antisense oligonucleotide）. VEGF and its receptor Fh-1 mRNA expression changes were detected by using real-time fluorescence quantitative detection after 24, 36 and 48 h. Result] The expression of VEGF mRNA in group Ⅰ, group Ⅱwere insignificantly different at higher expression levels, and did not change significantly with the time; the expression of VEGF mRNA in group Ⅲ assumed a slight decrease, but there were no significant differences between group Ⅰ and group Ⅱ （P 〉 0.05 ）, the expression of VEGF mRNA in group Ⅳ （ antisense oligonucleotide group ） decreased significantly （ P 〈 0.05 ） ; the expression of VEGF receptor FLT-1 mRNA was similar to that of VEGF Conclusion ] Antisense inhibition ghrelin has a downward effect to the expression of VEGF and its receptor Fh-1. |
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| Keywords: | Ghrelin Vascular endothelial growth factor Receptors Oligonucleotides Antisense |
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