Microsatellite markers and genetic diversity assessment in Lolium temulentum |
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| Authors: | Francis M Kirigwi John C Zwonitzer M A Rouf Mian Zeng-Yu Wang Malay C Saha |
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| Institution: | (1) Forage Improvement Division, The Samuel Roberts Noble Foundation, Inc., 2510 Sam Noble Parkway, Ardmore, OK 73401, USA;(2) Department of Plant Pathology, North Carolina State University, Gardner Hall No. 2518, Campus Box 7616, Raleigh, NC 27695, USA;(3) Corn and Soybean Research Unit, USDA-ARS, Ohio State University, OARDC, Wooster, OH 44691, USA |
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| Abstract: | Lolium and Festuca are two important genera of cool-season forage and turf grasses worldwide. Lolium temulentum L. (darnel ryegrass) has been proposed as a model species for genomics studies of cool-season forage and turf grasses. A
study with 41 darnel ryegrass, three tall fescue (Festuca arundinacea Schreb.), two tetraploid fescue (F. glaucescens), and two meadow fescue (F. pratensis) genotypes was initiated to (i) identify a set of microsatellite (simple sequence repeats) markers useful for L. temulentum L., and (ii) to utilize such markers for assessing the genetic variability of L. temulentum accessions collected from different geographical regions of the world. A total of 40 tall fescue (TF) EST-SSRs and 60 Festuca–Lolium (F × L) genomic SSRs were screened on a subset of eight genotypes. The selected 30 tall fescue EST-SSRs and 32 F × L genomic
SSRs were used for further analysis of genotypes. The TF-EST- and the F × L genomic-SSRs identified 10.3 and 9.3 alleles per
marker, respectively with an average polymorphic information content (PIC) value of 0.66. The phenogram based on 319 EST-SSR
and 296 genomic SSR fragments, grouped L. temulentum accessions into three major clusters except for accession ABY-BA 8892.78. Lolium temulentum accession ABY-BA 8892.78 did not cluster with any other accession. The Festuca clusters were distantly related with darnel ryegrass clusters with a similarity coefficient of 0.26. The selected set of
tall fescue EST- and F × L genomic SSRs were useful in assessing L. temulentum genetic diversity and could benefit the genetic improvement of members of the Festuca–Lolium complex. |
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| Keywords: | Festuca spp Genetic diversity Lolium temulentum Microsatellite markers |
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