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家猫超数排卵的研究
引用本文:吕连升,隋顺霞.家猫超数排卵的研究[J].畜牧兽医学报,1990,21(3):203-207.
作者姓名:吕连升  隋顺霞
作者单位:山东师范大学生物学系 (吕连升,隋顺霞,王新明,李兴诗),山东师范大学生物学系(郑洪霞)
摘    要:用促卵泡激素(FSH),孕马血清促性腺激素(PMSG)和人绒毛膜促性腺激素(HCG)建立家猫有效的超数排卵方法。FSH与PMSG的超排效果相比,前者明显优于后者。FSH 50单位/只的超排效果最好,最多者可达23个胚胎。胚胎回收率为68.18%。受精后前4天在输卵管可收集到1—、2—和4—细胞胚胎,第5天以后可在于宫收集到8—、16—细胞胚胎,第6天开始收集到桑椹胚。

关 键 词:  超数排卵  胚胎  FSH  PMSG

STUDIES ON SUPEROVULATION IN CATS
La Liansheng,Sui Shunxia,Wang Xinming,Li xingshi,Zheng Hongxia.STUDIES ON SUPEROVULATION IN CATS[J].Acta Veterinaria et Zootechnica Sinica,1990,21(3):203-207.
Authors:La Liansheng  Sui Shunxia  Wang Xinming  Li xingshi  Zheng Hongxia
Abstract:Fifty nine cats ( 39 females, 20 males ) were used in this study. Su-perovulation was induced by injection intramuscular of different dosages of FSH twice daily for 3 days or PMSG once, and then injection intravenous of HCG 100 IU/cat on the 4th day. The cats were mated or artificially inseminated with undiluted semen at the time HCG administered. The ovaries were examined and the oviducts and uteri were flushed in vitro at laparotomy for embryo recovery. The results were as follows. In comparison with PMSG, FSH is more efficient; The optimal dosage of FSH for superovulation of the adult cats is 50 IU/cat; The embryo recovery rate was 68.18%) 1-, 2-and 4-cell stages of embryos were collected from oviducts at the first four days after insemination, 8- and 16- cell stages of embryos from uteri on day 5 . The morulae were collected on day 6 .
Keywords:Superorulation  Embryo  FSH  PMSG  Cat
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