| 试纸条法和PCR法检测抗草甘膦转基因大豆的外源基因 |
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| 引用本文： | 阚贵珍,喻德跃.试纸条法和PCR法检测抗草甘膦转基因大豆的外源基因[J].中国油料作物学报,2005,27(4):18-21. |
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| 作者姓名： | 阚贵珍 喻德跃 |
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| 作者单位： | 1. 南京农业大学国家转基因大豆检测与监测中心,国家大豆改良中心,江苏,南京,210095
2. 南京农业大学作物遗传与种质创新国家重点实验室,江苏,南京,210095 |
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| 基金项目： | 国家转基因植物研究和产业化专项基金，引进国际先进农业科技计划(948计划)，江苏省国际合作项目 |
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| 摘 要： | 分别以转基因抗草甘膦大豆及其受体材料为阳性对照和阴性对照,对喷施除草剂草甘膦后部分存活的5个常规大豆材料后代进行试纸条检测和PCR检测.结果发现2种方法均在被检大豆材料中检测到抗草甘膦基因CP4 EPSPS,而且这2种方法的检测结果能相互验证;对这2种检测技术的应用前景进行了讨论.
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| 关 键 词： | 抗草甘膦大豆 外源基因 试纸条检测 PCR检测 |
| 文章编号： | 1007-9084（2005）04-0018-04 |
| 收稿时间： | 2005-03-27 |
| 修稿时间： | 2005年3月27日 |
Detection of trans-genes resistant to glyphosate in soybean by combining test strip and PCR methods |
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| KAN Gui-zhen,YU De-yue.Detection of trans-genes resistant to glyphosate in soybean by combining test strip and PCR methods[J].Chinese Journal of Oil Crop Sciences,2005,27(4):18-21. |
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| Authors: | KAN Gui-zhen YU De-yue |
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| Institution: | National Center for Detection and Supervision of Transgenic Soybeans, National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanfing 210095, China |
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| Abstract: | Using a soybean transgenic line which is resistant to glyphosate and its receptor material as positive control and negative control respectively, five soybean culti of glyphosate were examined by both the test strip and PCR vars partially methods. Th survived after the treatment with spraying e results showed that the CP4 EPSPS gene was detected in these soybean materials by both methods, at either protein or DNA level. And the test strip detection was consistent with PCR detection. Further application of these two techniques was discussed. |
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| Keywords: | Soybean with resistance to glyphosate trans-genes ELISA test strip PCR |
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