Advantages of a soluble dye in blot immunobinding assays for virus detection in potatoes |
| |
| Authors: | R W Gibson |
| |
| Institution: | (1) Rothamsted Experimental Station, AFRC Institute of Arable Crops Research, AL5 2JQ Harpenden, Herts, UK |
| |
| Abstract: | Summary A technique is described in which plant sap is blotted onto small pieces (8×11 mm) of nylon membrane and virus particles bound
to the paper are detected by a modification of the enzymelinked immunosorbent assay (ELISA). The detectable product of the
assay is a soluble yellow dye, the absorbance of which increased with the virus content of the plant sap. Leaf or tuber sap
from plants secondarily-infected with either potato leafroll virus or potato virus Y could be clearly distinguished from that
of healthy plants and a majority of tubers primarily infected with PVY were also detected. |
| |
| Keywords: | potato leafroll virus potato virus Y virus diagnosis |
| 本文献已被 SpringerLink 等数据库收录！ |
