| 贝利氏相思的组织培养和快速繁殖 |
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| 引用本文： | 张祖荣.贝利氏相思的组织培养和快速繁殖[J].西南农业大学学报,2007,29(3):86-89. |
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| 作者姓名： | 张祖荣 |
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| 作者单位： | 重庆文理学院生命科学系,重庆永川402168 |
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| 基金项目： | 重庆市教委科研资助项目（渝教科200404）《三峡库区观赏植物资源调查及快繁技术研究》的部分内容 |
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| 摘 要： | 用贝利氏相思茎段作外植体，采用在木本植物茎叶组培材料上经常采用的3种消毒方法，以及前人已在相思类植物营养器官组培上取得成功的4种芽诱导培养基、芽增殖培养基和生根培养基进行对比试验．结果表明，3种消毒方法效果都不错，只是75％酒精10s＋0．1％HgCl2 8min处理出现少量污染；芽诱导以改良MS＋6-BA 2．0＋NAA0．2效果最好；芽增殖以改良MS＋6-BA 1.0＋NAA0．2效果最好；生根效果最好的培养基是1／2MS十IBA1.0．
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| 关 键 词： | 贝利氏相思 茎段 组织培养 快速繁殖 |
| 文章编号： | 1000-2642（2007）03-0086-04 |
| 修稿时间： | 2006-05-20 |
Tissue Culture and Rapid Propagation of Acacia baileyana |
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| ZHANG Zu-rong.Tissue Culture and Rapid Propagation of Acacia baileyana[J].Journal of Southwest Agricultural University,2007,29(3):86-89. |
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| Authors: | ZHANG Zu-rong |
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| Institution: | Department of Life Science, Chongqing University of Arts and Sciences, Yongchuan, Chongqing 402168, China |
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| Abstract: | In this study,stem segments of Acacia baileyana F.J.Muell were used as explants in tissue culture.Three disinfection methods commonly used in the tissue culture of wooden plant stems and leaves and four bud abducting culture media,bud proliferating culture media and rooting media used by previous researchers in the tissue culture of vegetative organs of Acacia species were taken as a comparative test.The results showed that the effects of the three disinfection methods were fairly effective.A little mould occurred only on the stem segment treated with 75% alcohol for 10s + 0.1% HgCl2 for 8 min.The best medium for bud induction was a modified MS medium supplemented with 6-BA2.0 mg/L and NAA0.2 mg/L;the best medium for proliferation was MS+6-NBA1.0 mg/L+NAA0.2 mg/L and the best medium for rooting was a modified 1/2MS supplemented with IBA1.0 mg/L. |
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| Keywords: | Acacia baileyana stem segment tissue culture rapid propagation |
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