| 伪狂犬病病毒贵州分离株gE基因主要抗原表位区真核表达研究 |
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| 引用本文： | 郝飞,汤德元,李春燕,曾智勇,罗险峰.伪狂犬病病毒贵州分离株gE基因主要抗原表位区真核表达研究[J].贵州大学学报(农业与生物科学版),2013(6):484-488. |
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| 作者姓名： | 郝飞 汤德元 李春燕 曾智勇 罗险峰 |
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| 作者单位： | [1]贵州大学动物科学学院,贵州贵阳550025 [2]贵州省动物疫病预防控制中心,贵州贵阳550008 |
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| 基金项目： | 贵州省科学技术基金项目“猪呼吸道病毒性疫病新型特异诊断技术多重PCR检测的研究”[黔科合J字（2013）2110号];贵州省2011年农业攻关项目“贵州省规模化猪场主要病毒性疫病的调查及综合防控对策的研究与示范”[黔科合NY字（2011）3103号];贵州省2010年农业攻关项目“贵州省规模化猪场猪繁殖障碍性疫病防控体系的研究与示范”[黔科合NY字（2010）3042号] |
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| 摘 要： | 收集贵州省思南县某猪场送检的哺乳仔猪病料，采用Vero细胞盲传、核酸鉴定及测序分离到1株伪狂犬病病毒贵州分离株。扩增其gE基因主要抗原表位区，构建pcDNA3．1（＋）－gE重组真核表达质粒，将其转染Vero细胞，采用间接免疫荧光（IFA）检测基因在Vero细胞中的表达，同时将重组质粒免疫BALB／c小鼠，进行免疫学评价。结果表明：萨基因主要抗原表位区在Vero获得瞬时表达，转染的Vero细胞呈特异性绿色荧光；将构建的重组真核表达质粒免疫BALB／c小鼠，经间接ELISA和T淋巴细胞亚群含量测定表明，重组质粒具有较好的免疫原性。
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| 关 键 词： | 伪狂犬病病毒 贵州分离株 真核表达 |
Eukaryotic Expression of Main Epitope Domain of gE gene of PRV Guizhou Isolate |
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| HAO Fei,TANG De-yuan,LI Chun-yan,ZENG Zhi-yong,LUO Xian-feng.Eukaryotic Expression of Main Epitope Domain of gE gene of PRV Guizhou Isolate[J].Journal of Mountain Agriculture & Biology,2013(6):484-488. |
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| Authors: | HAO Fei TANG De-yuan LI Chun-yan ZENG Zhi-yong LUO Xian-feng |
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| Institution: | 1. College of Animal Sci- ence, Guizhou University, Guiyang 550025, Guizhou Province, P. R. China ; 2. Animal Disease Prevention and Control Center in Guizhou Province, Guiyang 550008, Guizhou Province, P. R. China) |
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| Abstract: | A pseudorabies virus （PRV） strain was isolated from piglets in Sinan County, Guizhou Province. To evaluate immune responses of the main epitope domain of PRV gE, the recombinant plasmid of pcDNA3.1 （ ＋ ） - gE was constructed and transfected into Vero cells. The expression of main epitope domain of gE was identified by indirect immunofluorescent assay. The results showed that main epitope domain of gE was transiently expressed in Vero cells and the protein expressed in Vero cells had good reactogenicity. Furthermore, BALB/c mice were immunized by pcDNA3.1 （ ＋ ） - gE. Indirect ELISA and determination of T lymphocyte subsets showed that the recombinant plasmid was efficiently able to induce immune responses in inoculated mice. The obtained evidences facilitated the further research on DNA vaccine against PRV. |
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| Keywords: | PRV Guizhou isolate Eukaryotic expression |
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