| 桑树根际硅酸盐细菌的分离鉴定及解钾能力测定 |
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| 引用本文： | 吴凡,刘训理,张楠,张莎莎,国辉,张本峰,仇念全.桑树根际硅酸盐细菌的分离鉴定及解钾能力测定[J].蚕业科学,2010,36(2):323-329. |
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| 作者姓名： | 吴凡 刘训理 张楠 张莎莎 国辉 张本峰 仇念全 |
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| 作者单位： | 山东农业大学林学院,山东泰安,271018;湖北省农业科学院经济作物研究所,湖北,武汉,430064;山东农业大学林学院,山东泰安,271018 |
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| 基金项目： | 山东省科技攻关计划项目 |
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| 摘 要： | 利用选择性培养基,从桑树根际土样中分离获得29个具有解钾能力的细菌分离株,经rep-PCR DNA指纹分析得到24株硅酸盐细菌。通过解钾能力测定,筛选出FK2、FK3、FK8、FK11、FK4′、FK23和PK187个具有较强解钾能力的菌株,其中FK2菌株的解钾能力最强,有效态钾增长41.79%。对这7株具有较强解钾能力的细菌进行菌落形态特征观察及16S rDNA序列测定和同源性分析:FK2菌株为假单胞菌属(Pseudomonas sp.),FK3和FK23菌株为中华根瘤菌属(Sinorhizobium sp.),FK11和FK8菌株为根瘤菌属(Rhizobiumsp.),FK4′菌株为中慢生根瘤菌属(Mesorhizobium sp.),PK18菌株为屈挠杆菌属(Flexibacter sp.)。
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| 关 键 词： | 桑树 硅酸盐细菌 rep-PCR DNA指纹分析 解钾能力 分类 菌落形态 16S rDNA |
Isolation and Identification of Mulberry Rhizospheric Silicate Bacteria and Determination of Their Potassium-releasing Activities |
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| WU Fan,LIU Xun-Li,ZHANG Nan,ZHANG Sha-Sha,GUO Hui,ZHANG Ben-Feng,QIU Nian-Quan.Isolation and Identification of Mulberry Rhizospheric Silicate Bacteria and Determination of Their Potassium-releasing Activities[J].Acta Sericologica Sinica,2010,36(2):323-329. |
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| Authors: | WU Fan LIU Xun-Li ZHANG Nan ZHANG Sha-Sha GUO Hui ZHANG Ben-Feng QIU Nian-Quan |
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| Institution: | WU Fan1,2 LIU Xun-Li1 ZHANG Nan1 ZHANG Sha-Sha1 GUO Hui1 ZHANG Ben-Feng1 QIU Nian-Quan1(1College of Forestry,Sh,ong Agricultural University,Taian Sh,ong 271018,China,2Industrial Crops Institute of Hubei Academy of Agricultural Sciences,Wuhan 430064,China) |
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| Abstract: | 29 bacterial isolates that had potassium-releasing activities were obtained from mulberry rhizospheric soil through cultivation with selective media.Analysis of repetitive-element PCR(rep-PCR)genomic DNA fingerprinting identified 24 silicate bacteria from them.Determination of their potassium-releasing activities indicated that 7 isolates,i.e.FK2,FK3,FK8,FK11,FK4',FK23 and PK18,had high potassium-releasing activities,among which FK2 had the highest potassium-releasing activity.Its available potassium increm... |
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| Keywords: | rep-PCR 16S rDNA |
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