A rapid procedure for screening the resistance of bean cultivars (Phaseolus vulgaris L.) to Botrytis cinerea and Sclerotinia sclerotiorum |
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| Authors: | G Leone A E G Tonneijck |
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| Institution: | (1) Research Institute for Plant Protection (IPO), P.O. Box 9060, 6700 GW Wageningen, The Netherlands |
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| Abstract: | Summary A method to inoculate detached bean leaves with Botrytis cinerea or Sclerotinia sclerotiorum without using a carbon-nitrogen source is described. Leaves were incubated in plastic trays that were covered with transparent plastic bags to maintain air humidity above 95%. Spores of either fungus inoculated in water alone were generally unable to cause infection. Additions of KH2PO4 (62.5 mM) or of mixtures of inorganic phosphate and glucose (1.1 or 5.5 mM) to the inoculum stimulated fungal pathogenicity. Also spore concentration (2×105 or 2× 106 spores ml–1) influenced the ability to produce lesions. At 2× 106 spores ml–1 both fungi were able to infect primary bean leaves when inoculated in an inorganic phosphate solution. These conditions were used for a preliminary screening for resistance of 15 bean cultivars to both fungi. Only partial resistance to the pathogens was found. Since the procedure is simple and does not require blooming plants, it can be rapidly adopted in screening and breeding programs.Abbreviations Pi
inorganic phosphate |
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| Keywords: | bean Botrytis cineres inoculation method Phaseolus vulgaris phosphate Sclerotinia sclerotiorum screening for resistance |
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