| 猪miR-206前体的克隆、表达及生物信息学分析 |
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| 引用本文： | 陈俭海,魏文娟,肖骁,赵书红.猪miR-206前体的克隆、表达及生物信息学分析[J].畜牧兽医学报,2007,38(5):425-431. |
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| 作者姓名： | 陈俭海 魏文娟 肖骁 赵书红 |
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| 作者单位： | 华中农业大学农业动物遗传育种与繁殖教育部重点实验室,农业部猪遗传育种重点开放实验室,武汉,430070 |
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| 基金项目： | 华中农业大学大学生科技创新基金项目（A06056）;国家自然科学基金（30672238）;国家重点基础研究发展规划（2006CB102105）湖北省自然科学基金创新群体项目（2006ABC008）资助.致谢 感谢本实验室周全勇、徐学文等在试验过程中给予的帮助和指导. |
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| 摘 要： | 研究骨骼肌中表达的miRNAs对深入探明骨骼肌生长发育的调控机制具有十分重要的意义。采用比较基因组学与生物信息学相结合的方法,从猪骨骼肌中克隆了猪miR-206的前体（Precursor）,对该miRNA前体的二级结构及与其它物种序列的同源性进行了分析。RT-PCR结果显示,该前体miRNA在猪大多数组织中均有表达,在骨骼肌中表达量最高。生物信息学分析表明miR-206前体序列在不同物种间具有较高的保守性。
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| 关 键 词： | 猪 miR-206前体 克隆 表达 |
| 文章编号： | 0366-6964（2007）05-0425-07 |
| 修稿时间： | 2007-01-11 |
Cloning,Expression and Bioinformatics Analysis of miR-206 Precursor in the Pig |
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| CHEN Jian-hai,WEI Wen-juan,XIAO Xiao,ZHAO Shu-hong.Cloning,Expression and Bioinformatics Analysis of miR-206 Precursor in the Pig[J].Acta Veterinaria et Zootechnica Sinica,2007,38(5):425-431. |
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| Authors: | CHEN Jian-hai WEI Wen-juan XIAO Xiao ZHAO Shu-hong |
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| Institution: | Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education ,Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070,China |
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| Abstract: | MiRNAs play important roles in skeletal muscle development. In this study, the porcine miR-206 precursor is cloned and sequenced from skeletal muscle through comparative genomics approach. The secondary structure was predicted. RT-PCR results indicated that the miR-206 precursor is expressed in most of the tissues and exhibited the highest expression level in skeletal muscle. Homology analysis revealed that pre-miR-206 is highly conserved among different species. |
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| Keywords: | pig miR-206 precursor cloning expression |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
