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酶法提取茶多糖工艺条件的研究
引用本文:郭艳红,魏新林,王元凤,张嘉辰.酶法提取茶多糖工艺条件的研究[J].农产品加工.学刊,2009(4).
作者姓名:郭艳红  魏新林  王元凤  张嘉辰
作者单位:上海师范大学,生命与环境科学学院,上海,200234
基金项目:国家高技术研究发展计划(863计划),上海市科技启明星计划,上海崇明生态岛建设重大科技专项基金,上海市教育委员会资助项目 
摘    要:用酶法提取茶叶多糖。研究酶种类、添加量、温度和pH值对多糖含量的影响,并在最佳工艺基础上探讨茶多糖得率,中性糖、酸性糖含量和蛋白质含量。结果表明,质量分数为0.8%的茶叶水解酶,在pH值5.5,温度48℃的条件下,茶多糖含量最高,得率为2.01%,中性糖含量为54.27%,酸性糖含量为15.41%,蛋白质含量为7.91%。

关 键 词:茶多糖    提取工艺

Study on Enzymatic Extraction Processing of Tea Polysaccharides
Cuo Yanhong,Wei Xinlin,Wang Yuanfeng,Zhang Jiachen.Study on Enzymatic Extraction Processing of Tea Polysaccharides[J].Nongchanpin Jlagong.Xuekan,2009(4).
Authors:Cuo Yanhong  Wei Xinlin  Wang Yuanfeng  Zhang Jiachen
Institution:College of Life and Enviroment Science;Shanghai Normal University;Shanghai 200234;China
Abstract:Tea polysaccharides were extracted by enzyme hydrolysis method.the effect of concentration,pH and temperature on content of polysaccharides was studied respectively.And the yield rate,neutral tea polysaccharides,acidic tea polysaccharies and protein content were studied.The optimum process conditions of enzymatic extraction technology were determined as following:the enzyme concentration,0.8%,pH 8.77;temperature 58.9 ℃.Under this condition,the yield rate reached to 2.01%,neutral tea polysaccharides content was 54.27%,acidic polysaccharides content was 15.41% and protein content was 7.91%.
Keywords:tea polysaccharides  enzymatic  extractionprocessing  
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