|
|||||
|
|
| 番茄组织总RNA提取方法研究 | |
| 引用本文： | 李大志,邓子牛,熊兴耀,谢丙炎.番茄组织总RNA提取方法研究[J].湖南农业大学学报(自然科学版),2007,33(5):572-575. |
| 作者姓名： | 李大志 邓子牛 熊兴耀 谢丙炎 |
| 作者单位： | 1. 湖南农业大学,园艺园林学院,湖南,长沙,410128;中国农业科学院,蔬菜花卉研究所,北京,100081 2. 湖南农业大学,园艺园林学院,湖南,长沙,410128 3. 中国农业科学院,蔬菜花卉研究所,北京,100081 |
| 摘 要： | 从RNA的完整性、纯度和产量等方面比较了TRIzol,RNAplant,TRIpure等3种RNA提取试剂对番茄不同组织部位总RNA的提取效果.结果表明,RNAplant能从成熟组织中获得较高质量和产量的总RNA,用该方法提取的番茄老化组织总RNA经RT-PCR能成功进行内参检测. |
| 关 键 词： | 番茄 总RNA RT-PCR |
| 文章编号： | 1007-1032（2007）05-0572-04 |
| 修稿时间： | 2007-06-25 |
Study on isolation of total RNA from tomato tissues |
|
| LI Da-zhi,DENG Zi-niu,XIONG Xing-yao,XIE Bing-yan.Study on isolation of total RNA from tomato tissues[J].Journal of Hunan Agricultural University,2007,33(5):572-575. | |
| Authors: | LI Da-zhi DENG Zi-niu XIONG Xing-yao XIE Bing-yan |
| Institution: | 1. College of Horticulture and Landscape, HNAU, Changsha 410128, China; 2.Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China |
| Abstract: | TRIzol Reagent,RNAplant and TRIpure Reagent were used for RNA extraction from 3 tomato tissues, including leaf,stem and root.Total RNA were evaluated upon quality and yields.RNAplant was found to be the best one among the 3 reagents evaluated.With this reagents,higher amount and quality of RNA could be successfully extracted from all 3 kinds of tissues tested.The RNA obtained from all 3 reagents were successfully used to amplify cDNA fragments by RT-PCR . |
| Keywords: | tomato total RNA RT-PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |