| 甘露聚糖酶基因Man23在芽孢杆菌WB600中的表达 |
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| 引用本文： | 胡杨,周海燕,董蕾,吴永尧.甘露聚糖酶基因Man23在芽孢杆菌WB600中的表达[J].湖南农业大学学报(自然科学版),2007,33(5):539-541. |
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| 作者姓名： | 胡杨 周海燕 董蕾 吴永尧 |
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| 作者单位： | 1. 湖南农业大学科学技术师范学院,湖南,长沙,410128
2. 湖南农业大学生化与发酵工程实验室,湖南,长沙,410128 |
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| 基金项目： | 湖南省科技计划重大专项项目 |
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| 摘 要： | 兼具纤维素酶和半纤维素酶活性的Man23基因(Man23)来自于芽孢杆菌B23,将其连接到质粒pHY-P43上,由强启动子P43来启动Man23的表达,重组质粒pHY-p43-Man23转入蛋白酶缺陷型芽孢杆菌WB600中,表达产物与原体系相比,甘露聚糖酶活力提高了21%,电泳检测得到的蛋白图谱显示目的酶丰度有显著提高,杂蛋白未见大量表达,可见构建的WB600体系适用于Man23基因表达.
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| 关 键 词： | 甘露聚糖酶 基因表达 芽孢杆菌WB600 |
| 文章编号： | 1007-1032（2007）05-0539-03 |
| 修稿时间： | 2007-07-09 |
Expression of recombinant mannanase gene Man23 in Bacillus sp. WB600 |
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| HU Yang,ZHOU Hai-yan,DONG Lei,WU Yong-yao.Expression of recombinant mannanase gene Man23 in Bacillus sp. WB600[J].Journal of Hunan Agricultural University,2007,33(5):539-541. |
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| Authors: | HU Yang ZHOU Hai-yan DONG Lei WU Yong-yao |
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| Institution: | a. Normal College of Science and Technology; b.Biochemistry and Fermentation Engineering Lab, HNAU, Changsha, 410128, China |
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| Abstract: | Enzyme Man23 can hydrolyze cellulose and hemicelluloses. In this paper,its gene was cloned and then ligated with plasmid pHY-p43.Recombinant plasmid pHY-p43-Man23 was transformed into Bacillus sp. WB600. Because of the stronger promoter p43 and proteinase deficient bacterium,the recombinant Man23 had mannanase activity about 21% higher than wild type.The electrophoretogram of WB600 system was cleaner and little non-target protein was determined.All the results showed that the WB600 system was suitable to be a high-level expression system of gene Man23. |
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| Keywords: | mannanase gene expression Bacillus sp WB600 |
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